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Objective: Hedgehog pathway inhibitor Cyclopamine, understanding in invasive breast cancer cells MDA -MB ??- 231 exists Hedgehog signaling pathway activation , to study and to explore the role of the Hedgehog signaling pathway in human breast cancer cells MDA-MB-231 invasion and metastasis , and mechanism. Methods : effective inhibitory concentration in human breast cancer cells MDA-MB-231 as the research object , using the the Hedgehog the pathway inhibitor Cyclopamine processing cells , MTT assay cell proliferation , to strike 48 hours . 50μmol / L Cyclopamine role of MDA-MB-231 cells , 48 hours , Transwell chamber invasion and migration assay of the Matrigel ( artificial reconstituted basement membrane ) ; the gelatin zymography cells secreted MMP -2 , MMP - 9 changes ; Western-blot Pc-Jun, MMP-9 and uPA protein expression in the cells was detected ; RT-PCR detection of Gli1 cells , c -jun , MMP -9 and uPA mRNA expression changes . The results : the application Hedgehog the pathway inhibitors Cyclopamine , significantly inhibited the proliferation of MDA-MB - 231 cells ( P lt; 0.05 ) , 48 hours in effective inhibitory concentration for 95.5μmol / L . 50μmol / the L of Cyclopamine role in cell 48 hours later , cell invasion , migration decreased ( P lt; 0.05 ) ; MMP -9 activity decreased (P lt; 0.01); Pc-Jun, MMP-9, uPA protein expression were reduced ( P lt; 0.01 ) ; of Gli1 , c - jun , MMP -9 and uPA mRNA expression reduce ( P lt ; 0.01) . Conclusion: human breast cancer cells MDA-MB-231 exist Hedgehog signal transduction pathway activation the activation Hegehog signal path through the regulation of c -jun , MMP -9 and uPA expression to promote breast cancer cell invasion and metastasis .
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