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Effect of Retinoic Acid on the Expression of Keratinocyte Growth Factor and Keratinocyte Growth Factor Receptor in the Injuried Lung Tissue of Neonatal Rats Exposed to Hyperoxia

Author: ZhangYaWei
Tutor: ChenZuoZuo
School: Chongqing Medical University
Course: Pediatrics
Keywords: High oxygen Retinoic acid Lung injury Keratinocyte growth factor Rats
CLC: R722.1
Type: Master's thesis
Year: 2011
Downloads: 0
Quote: 1
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Abstract


Objective To investigate the hyperoxia-exposed neonatal rat lung tissue changes in the structure and keratinocyte growth factor (KGF) and its receptor (KGFR) expression and retinoic acid (RA) on them. The method to be born within 24h 90 SD rats were randomly divided into three groups , I group: the air normal saline ( NS ) ; II group : high oxygen NS ; Group III high oxygen RA . Group II, III continuous exposure to 85 % oxygen , the group I placed in the air ; the Group III daily intraperitoneal injection of RA, I, II group received daily intraperitoneal injections NS . 4,7,14 d after birth of lung specimens the HE staining observed changes in the lung tissue structure and radial alveolar count ( RAC ) ; expression of RT - PCR detection of KGF and KGFRmRNA strength and immune histochemical assay KGF protein levels . Results after birth ( 1) 14d , II , III group compared with the group I RAC values ??significantly reduce ( P lt; 0.05 ) , III group was significantly higher than in group II ( P lt; 0.05 ) . (2) KGF mRNA expression of strength : compared with the group I and II group 4d , KGFmRNA expression significantly increased (P lt; 0.05); subsequently began to decline , the 7d but remained increased in group I ( P lt; 0.05 ) ; 14d III group at each time point expression were higher than in group II ( P lt; 0.05 ) compared with group I decreased ( P lt; 0.05 ) ; 4d , the the groups KGFRmRNA expression levels was no significant difference ; 7d , 14d , II, III group expression level was significantly lower than Group I ( P lt ; 0.05) , and no significant difference between Group II and Group III (P gt ; 0.05). (3) KGF - positive cells were mainly distributed in the part of the alveolar wall and alveolar surrounding vascular endothelial cells and mesenchymal cells . KGF protein expression intensity and its mRNA expression changes similar . Conclusion RA can promote KGF expression in lung tissue , improve induced by hyperoxia lung stunting . Of immature lung hyperoxic injury protection .

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CLC: > Medicine, health > Pediatrics > Newborns, premature children disease > Neonatal disease
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