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Expression of MIF and ICAM-1 in Severe Acute Pancreatitis Associated Lung Injury in Rat

Author: LiQiZhi
Tutor: WuXinMin；GuoYaMin
School: Qinghai University
Course: Surgery
Keywords: severe acute pancreatitis lung injury intercellular adhesion molecule 1 macrophage migration inhibitory factor myeloperoxidase
CLC: R576
Type: Master's thesis
Year: 2011
Downloads: 17
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Abstract

Objective To establish an severe acute pancreatitis animal model and study the role of the macrophage migration inhibitory factor (MIF) and intercellular adhesion molecule 1 (ICAM-1) at severe acute pancreatitis associated lung injury (SAPALI) in rat.Methods 60 specific pathogen free (SPF) wistar male rats were divided into five groups randomly: blank group (n=12), sham-operated group (n=12), six hours after SAP group (n=12), twelve hours after SAP group (n=12), twenty-four hours after SAP group (n=12). SAP was induced by injection of 5% Na-Tc under membrane of pancreas. The rats were killed at sixth hour after operated in Sham-operated group. The rats were killed at sixth, twelfth and twenty-fourth hour after Na-Tc injection in six hours after SAP group, twelve hours after SAP group and twenty-four hours after SAP group respectively. The content of amylase in serum was measured by holo-automatic biochemical analysator. Pancreatic and pulmonary tissues were observed by hematoxylin-eosin (HE) staining, and were counted for the pancreatic tissues and histological scores and pulmonary histopathologic scores. Appling Blood-gas Analyzer determination PO2 and PCO2.Detecting the concentration of soluble intercellular adhesion molecules-1 (sICAM-1) in serum with ELISA method. Detecting the expression of intercellular adhesion molecules-1 (ICAM-1) and macrophage migration inhibitory factor(MIF)in pancreatic tissue and pulmonary tissue by immuno- histochemical method. Detecting the pulmonary Myeloperoxidase (MPO) activety. All the data were analyzed by statistics software SPSS18.0. Normal distribution measurement data was shown as means±standard deviation ( x±s). One-way ANOVA method was applied to comparison among groups. And using S-N-K method for binary comparison. Pearson correlation analysis was applied to measure the relevant relations. The inspection standards was alpha = 0.05.Results We successfully established severe acute pancreatitis animal model. The rats of SAP group exhibited a high amylase, severe pancreatic pathological damage and pulmonary pathological damage after modeling. The index of MIF and ICAM-1 in Sham-operated group compared with Blank group was no significant difference (P>0.05). The index of MIF and ICAM-1 in six hours after SAP group, twelve hours after SAP group and twenty-four hours after SAP group compared with blank group and sham-operated group was significant difference(P<0.01), and six hours after SAP group, twelve hours after SAP group and twenty-four hours after SAP group compared with each other was significant difference(P<0.05). The content of MPO activity and sICAM-1 in sham-operated group compared with blank group was no significant difference (P>0.05). The content of MPO activity and sICAM-1 in six hours after SAP group, twelve hours after SAP group and twenty-four hours after SAP group compared with blank group and sham-operated group was significant difference (P<0.05), and six hours after pancreatitis group, twelve hours after pancreatitis group and twenty-four hours after pancreatitis group compared with each other was significant difference(P<0.05). Pancreatic histopathologic scores and pulmonary histopathologic scores were highly relevant existing (r>0.7).The content of sICAM-1, MIF,ICAM-1 and MPO horizontal were highly related (r>0.7) with the content of pulmonary histopathologic scores.Conclusions①In the severe acute pancreatitis associated lung injury, the lung injured degree is closely related to the degree of the pancreatic injury.②In the severe acute pancreatitis associated lung injury, the expression of MIF is positively correlated with the lung injured degree.③In the severe acute pancreatitis associated lung injury, the expression of ICAM-1 is positively correlated with the lung injured degree.

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