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Comparative Study on Beagle’s LAD Branches Angle of Subepicardial Intra-myocardium Coronary Artery between Ultrasonograph and Histopathology

Author: FuYing
Tutor: YinLiXue
School: North Sichuan Medical College
Course: Medical Imaging and Nuclear Medicine
Keywords: Acoustic cardiography Coronary Branch Angle Subepicardial myocardial
CLC: R445.1
Type: Master's thesis
Year: 2011
Downloads: 6
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Abstract

Objective: application of high-frequency two-dimensional gray-scale ultrasound, color Doppler flow imaging (color Doppler flow imaging, CDFI) and myocardial contrast echocardiography (myocardial contrast echocardiography, MCE) imaging technique to observe health than the Beagles left anterior descending the branch (left anterior descending coronary artery, LAD) epicardial myocardial walk the line characteristics and regional heart measurement LAD trunk and epicardial branch bevel branch (oblique branch, OB) traveling on the high-frequency two-dimensional ultrasound grayscale image epicardial coronary (epicardial coronary artery, EPCA) to its epicardial intramyocardial coronary artery (subepicardial intra-myocardium coronary artery, SIMCA) branch point angle (branches angles, BA), and the pathologic specimens with heart microscope photography image measurement (the BRANCHES Angles of the epicardial-subepicardial Intra-AND CYTOCHEMISTRY CORONARY ARTERY, in EP-SIMCA-BA) compared. Torsional motion biomechanical theory is based on fluid dynamics and heart, investigate EP-SIMCA-BA characteristics and the relationship of the coronary circulation, reveal myocardial perfusion physiological characteristics. Try to establish the evaluation of left ventricular vivo coronary ultrasound evaluation system build. Methods: Transcatheter the outer membrane echocardiography get eight healthy thoracotomy than the Beagles LAD trunk and its branches high frequency two-dimensional gray-scale ultrasound, CDFI and MCE dynamic sonogram. The epicardial myocardial observe the LAD and its main branch direction of travel trunk in the LAD, the last paragraph of the bevel branch (the superior segment of oblique branch SSOB) and under paragraph (inferior segment of the oblique branch ISOB) in central and apical level were measured different ultrasound technology acquisition EP-SIMCA-BA. Image acquisition after eight beagle dogs were randomly divided into two groups of four each. Different experimental group dogs were sacrificed with BaCL2 and KCL solution, so that the first group of dogs cardiac arrest in systolic, Group 2 dogs cardiac arrest in end-diastolic. Fixed heart specimens, take the left ventricular anterior wall along the LAD trunk and its branches histopathology; intramyocardial LAD epicardial walk the line characteristics under the microscope, and the determination of LAD, SSOB, traveling area ISOB EP-SIMCA -BA. Measured on the high-frequency two-dimensional ultrasound grayscale image EP-SIMCA-BA and EP-SIMCA-BA, Gross specimen microscope photographic image as measured using the Pearson correlation analysis of binding Bland-Altman analysis of each image as measured LAD single factor analysis of variance and LSD method test the SSOB and ISOB go row area between the three EP-SIMCA-BA, the same walk the line regional end-diastolic and end-systolic EP-SIMCA-BA compared using paired t-test, two different operating Get data reproducibility were evaluated using Pearson correlation analysis and Bland-Altman method analysis. Results: two-dimensional gray-scale ultrasound, color Doppler flow imaging, myocardial contrast echocardiography, biopsy specimens and tissue under a microscope qualitative observation: LAD Beagles more acute angle to the branch within the lower epicardial myocardial screwed \intramyocardial branch near right angles to their superiors EPCA. Gross and histopathological microscope internal elastic membrane thickening in the coronary artery branches protrude into the lumen formation of intimal cushion. Two-dimensional gray-scale ultrasound, endoscopic photography image quantitative evaluation of gross specimen: ⑴ long axis view of the two-dimensional ultrasound grayscale images on LAD single-factor analysis of variance among the EP-SIMCA-BA SSOB, and ISOB walk line area, the difference statistically significant (P lt; 0.01), pairwise comparisons were statistically significant differences (P lt; 0.01); including LAD gt; SSOB gt; ISOB. Running ISOB EP-SIMCA-BA area the θd greater than θs, the difference was statistically significant (P lt; 0.01); while the EP-SIMCA-BA area of ??the LAD and SSOB traveling θd and θs compare the difference was not statistically significant (P gt; 0.05). (2) long axis view the gross specimen on the microscope photography images LAD, among SSOB and row area of ??ISOB go EP-SIMCA-BA single-factor analysis of variance, the difference was statistically significant (P lt; 0.001) the LAD and SSOB respectively with ISOB comparison, the difference was statistically significant (P lt; 0.001) the LAD and SSOB difference between statistical significance (P gt; 0.05); the LAD, SSOB and ISOB go line area EP-SIMCA-BA θd and θs were not different statistically significant (P gt; 0.05). ⑶ two-dimensional ultrasound gray-scale and gross specimen microscope images are two ways measured the walk line area of ??the LAD, SSOB and ISOB EP-SIMCA-BA analysis, the difference was not statistically significant (P gt; 0.05). EP-SIMCA-BA results are not consistent; measured in two ways using the Bland-Altman analysis, the only end-systolic LAD, of LAD and ISOB end diastolic two ways to measure the average difference of EP-SIMCA-BA substantially consistency trends. ⑷ repetitive inspection: randomly selected two operators on the measured data in the two-dimensional ultrasound grayscale image correlation analysis LAD, SSOB, go row area ISOB EP-SIMCA-BA were compared, with a high correlation (r = 0.76 ~ 0.92, P lt; 0.001 or P lt; 0.05), randomly selected from two operations by two-dimensional ultrasound grayscale images and specimens microscope images LAD end diastolic EP-SIMCA-BA measured values ??Bland-Altman analysis , Bland-Altman diagram shows two operator repeated measure the the EP-SIMCA-BA difference and mean consistency on the trends. Conclusion: 1. Epicardial coronary artery was acute angle into the epicardial myocardial angle gradually decreases from the proximal to the distal, branch distal angle cardiac cycle of the branch's change is obvious. Coronary branch acute angle connection \2. Coronary artery wall structure and its rotation into lower epicardial myocardium and with myocardial rotation rotating out of shape features consistent with ventricular wall biomechanical properties twisting motion. 3 high-frequency two-dimensional gray-scale ultrasound, color Doppler flow imaging, and myocardial ultrasound imaging techniques can accurately reveal coronary artery into the lower epicardial myocardial branch angle characteristics, and real-time display of the coronary branch angle changes with heart systolic and diastolic movement of time and space. 4 of this study will help to further understanding of the characteristics of the myocardial perfusion, further human coronary circulation ultrasound studies provide the basis of experimental evidence and methods, and to provide the basis of the anatomical structure information for clinical evaluation and treatment of coronary artery disease.

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