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[Objective] A Comparative Study of the similarities and differences between Vietnam and China Guangdong produced patchouli volatile and flavonoid fraction composition of the similarities and differences of the comparative introduction and cultivation of its intrinsic quality, for patchouli cultivation of planting to provide a reference; through a comparative study of different origin processing and the quality of the cut process patchouli, preferably appropriate of Origin processing methods and cutting process, the quality of patchouli Pieces standardized to provide scientific basis. [Methods] multiple batches from Vietnam and China Guangdong patchouli, extracted by steam distillation of volatile components, each sample component is detected by GC-MS, comparing its components and contents of the similarities and differences. Apigenin and rutin reference substance, to establish the patchouli total flavone by spectrophotometry using HPLC method A Comparative Study of the methanol extract fractions and the similarities and differences of the flavonoid fraction fingerprint explore two Origin patchouli non-volatile The components of the correlation. Reference to of patchouli traditional processing method, and incorporated into the processing methods may be the main factors affecting the composition changes, design Origin and cutting process, volatile oil and total flavonoids assessment indicators, the preferred suitable origin processing methods and the cutting process. [Results] After the comparison of the two countries patchouli volatile components of fingerprinting analysis, obtained fingerprint similarity is lower, respectively, Guangzhou, China 0.8018, Zhanjiang, Guangdong 0.8458, Guangdong GAOYAO, 0.9008, Guangdong To Shin Pharmaceutical 0.8678, Vietnam Hanoi 0.8345, Vinh 0.8800, 0.9154 Son La, Hanoi 0.9207, Hanoi 0.8666, Viet Tri 0.9126, Zhanjiang GAP base of 0.7746, 0.7650 the Zhanjiang GAP base, multi-component sub-content difference between the larger, Vietnamese patchouli volatile oil content of 1.52% in Hanoi, Vinh 1.38% La, 1.89%, 2.07% Hanoi, Hanoi, 2.16%, the Viet Tri 1.38%; generally higher than production patchouli, Guangzhou, Guangdong, China, 1.32%, 1.43 percent in Zhanjiang, Guangdong, Guangdong Gaoyao 1.71%, 1.02% sent a letter to the Pharmaceutical in Guangdong. Total flavonoid content results were as follows: 0.82% Chinese, Vietnamese 0.95% (apigenin); 0.71% 0.78%, Vietnam (rutin). The the total flavonoids parts of Vietnam and China from different sources patchouli herbs HPLC fingerprint initially established 13 common peaks characteristic peaks of its similarity to Guangzhou, China 0.9450, Zhanjiang, Guangdong 0.9448, Guangdong GAOYAO 0.8924, Guangdong sent a letter to Pharmaceutical 0.9543, Hanoi, Vietnam, 0.9449, the Vinh 0.9513, Son La 0.8770, Hanoi 0.9377, Hanoi 0.8772, Viet Tri 0.8782, Zhanjiang GAP base 0.6411, Zhanjiang GAP base 0.5593. Different sources medicinal parts of methanol fingerprint similarity Guangzhou, China 0.9631, Guangdong Zhanjiang 0.9451, Guangdong Gaoyao 0.9342, Guangdong sent a letter to the Pharmaceutical 0.8912, Hanoi, Vietnam, 0.8439 Wing City, 0.9213, mountain radish 0.8673 Hanoi 0.9377, Hanoi 0.8772, the more the pool the 0.8782, Zhanjiang GAP base 0.762, Zhanjiang GAP base of 0.7593. Method of processing and cutting process 2 content of volatile oil was rate to 1.20% ± 0.01%, the highest significant the difference, so the most suitable for the increase in the content of volatile oil of the original processing method and cutting process is: method 1, process 2, that is to take The heap stew dried in the sun during the day and at night, to dry, and then per kilogram of herbs touch spilled 200 mL of water infiltration for 20 min, cut into inch segments, that was patchouli Pieces. [Conclusion] produced in Vietnam patchouli volatile oil content is higher than the the patchouli volatile oil content; has a certain similarity of of patchouli two volatile components in the component, but the content of each component more than there is a significant difference, introduction to China main active parts of the content of patchouli larger changes. Using apigenin as a reference substance, purified by polyamide according to the UV spectrophotometric determination of patchouli total flavonoids method is simple and fast, a good linear relationship, sensitivity and accuracy, apply to the the patchouli total flavonoid content was measured directly. The test showed that Vietnamese patchouli total flavonoid content was significantly higher than China's introduction. Created the patchouli total flavonoids HPLC fingerprint method feasible, reproducible, and can be used as parts of the evaluation of the quality of patchouli herbs flavonoids and identification of indicators. The relative content of the different sources patchouli total flavonoids fingerprint characteristic peaks there are some differences. After the The patchouli introduction to China's Guangdong, due to the impact of the growth environment, the main active site and the active ingredient content change significantly. Patchouli processing of origin need to strictly control the operation of the sweating process, the process of cutting its volatile oil and total flavonoids.
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