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Study on the Pharmacodynamics and Toxicity Test of the Feng Shi Tong Xiao Pill

Author: XiaoJinYu
Tutor: WangBingSheng;ZhangDanCan
School: Hebei North University
Course: Pharmacology
Keywords: Rheumatism Pills Anti-inflammatory Cytokines Synovial lesions Toxicity
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 34
Quote: 0
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Abstract


Five cytokines in the experimental research rheumatism Pills, anti-inflammatory and analgesic effects of adjuvant arthritis (AA) rat serum IL-1, IL-4, IL-10, the RF, TNF-α regulation, reduce synovial lesions and long-term toxicity. Take 200 Kunming mice, Wistar rats 140, in SPF grade laboratory normal 10 days after the feeding experiment. The antiinflammatory experimental xylene induced ear edema model; analgesic experiments with hot plate, radiant heat irradiation method and acetic acid writhing body law model. The four models, each of 50 mice were divided into blank control group, low-dose group, middle dose group, high dose group, aspirin positive control group, n = 10, all the hot plate method using female mice, the remaining three groups of small mouse male and female experiment; 60 rats were randomly divided into blank control group, model group, the treatment group, Tripterygium glycosides group model 30, 10 of the remaining three groups, male and female, Eph. 's complete adjuvant per the model only 0.1mL injection in the treatment group, Tripterygium polyglycosidium rats right hind paw, modeling the first two weeks of the 10 rats kill the model, take ankle light microscope to observe the pathological changes of the synovium, blood was collected at the same time, using the enzyme-linked immunosorbent assay (ELISA) in the detection of serum IL-1, IL-4, IL-10, RF, TNF-α five kinds cytokine levels. Modeling 15d, blank control group, the model remaining rats given 5% sodium carboxymethyl cellulose solution 2mL orally, the treatment group was given (the concentration of 0.075g · mL -1 ) rheumatoid the pain the elimination pills suspension gavage, Tripterygium glycosides group was given the (concentration of 0.005g · mL -1 ) Tripterygium suspension orally administered volume are 2mL twice a day, in the morning, 8:00, 20:00 administration. Administration 15d after blood collection, using enzyme-linked immunosorbent assay (ELISA) to detect serum IL-1, IL-4, IL -1 , RF, TNF-α five cytokines of level. Take the rat ankle light microscopy pathological changes of the synovium. The long-term toxicity tests, take the remaining 80 rats were randomly divided into blank control group, the high-dose group, middle dose group and low dose group, n = 20. 8g · mL -1 , 4g · mL -1 , 1g · mL -1 , respectively, of the high-dose group, middle dose group, low dose group were gavage, volume 2mL, twice daily. The blank group was given 5% sodium carboxymethyl cellulose solution as a control. Continuous administration of three months, 10 rats from each group to do blood testing blood, take the kidney, liver, stomach, histopathological detection. The recovery remaining 40 rats feeding, do the same in the two weeks after. In the anti-inflammatory and analgesic experiments, the middle dose group of mice ear edema inhibition rate pain threshold percentage writhing inhibition rate with blank control group, low-dose group, high dose group showed a significant difference (P lt; 0.05), compared with the aspirin group, no significant difference (P gt; 0.05). Treatment serum IL-1, RF, TNF-α levels with model significantly lower (P lt; 0.01), IL-4, IL-10 level was significantly higher (P lt; 0.05) synovial lesions significant change before and after administration Tripterygium glycosides group showed no significant difference (P gt; 0.05). Toxicity testing, drug rats routine blood test indicators with blank group significant sex difference (P gt; 0.05), and pathological examination of rat kidney, liver, stomach and other organs and tissues were not found to have significant toxicity damage changes. Pills has significant anti-inflammatory and analgesic effects of rheumatic pain, a therapeutic effect on the pathological changes of the synovium, and on the role of the five cytokines in the serum of AA rats. Taking higher doses and longer treatment of rheumatism Pills low toxicity, taking safety, better treatment of rheumatoid arthritis in clinical compound Chinese medicine.

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