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Protein Architectures as Molecular Fossils to Trace Metabolic Evolution

Author: QinTao
Tutor: ZhangHongYu
School: Shandong University of Technology
Course: Biochemistry and Molecular Biology
Keywords: Protein Structure Molecular fossils Biosynthesis Metabolic evolution
CLC: Q51
Type: Master's thesis
Year: 2011
Downloads: 40
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Abstract

In life science research, protein is one of the important research objects. Constantly enrich the protein structure data, the study of the evolution of protein structure and function has achieved great development, especially the evolution of enzyme structure and function. Vivo chemical reactions are almost always carried out in the catalytic action of the enzyme for the evolutionary history of these reactions, however, is still unknown. Therefore, this thesis build-based protein folding type (F) superfamily (FSF) and family (FF) system tree to track the biochemical reactions in the metabolism of evolutionary events. Evolution of the overall metabolism, amino acid biosynthesis, inorganic chemical evolution of aerobic metabolism evolution and metabolic network evolution and other aspects of research, the protein spatial structure can be used as molecular fossils to study the origin and evolution of modern biochemistry, in order to further understand life evolution the essence of a meaningful clues, but also shows the potential of the method for tracking other evolutionary events. Studies have shown that protein folding type (F) and superfamily (FSF) molecular clock can be traced biochemistry evolutionary events. Cysteine ??and histidine with a variety of biochemical function, plays a vital role for the entire life. The use of the newly created protein F and FSF molecular clock to infer cysteine ??and histidine biosynthetic origin time of approximately 33 to 35 million years ago, the discovery contributed to a better understanding of the nature of early life. Biochemical reactions of sulfur-containing inorganic preliminary study found that the early reaction is essentially related to cysteine ??metabolism, and cysteine ??assimilation and catabolism is almost the same time (about 36.8 million years ago), and then only the sulfate reduction reaction, the first occurrence of oxygen until about 29 to 30 billion years ago, and after the reaction are essentially within the oxidation reaction of sulfuric acid. In addition, it is also found that the assimilation and dissimilation sulfate reduction pathway appears earlier, and concluded that generate sulfide reaction in the 25.1 billion years ago, a certain significance of these results for the in-depth study of the evolution of sulfur. Relative to the protein F or FSF, protein FF and function more closely linked. Depth protein FF in protein F and FSF molecules of minutes, as a molecular clock to infer the evolution of biochemical reactions. First early FF (Nd-LT; 0.2) mainly relates to the primary metabolism, purine and pyrimidine metabolism appears first, followed by the amino acids, sugars, cofactors, terpene compounds, glycerol and porphyrin metabolism, further confirmed metabolic origin of the energy conversion reaction of the nucleotide metabolism. In the same time, the early FF corresponding to the pathway shows the evolutionary sequence of amino acid biosynthesis, is consistent with other methods deduced amino acid corresponding to codon chronological. Second, identified by the oldest enzyme the ATP phosphate hydrolase (EC 3.6.3.49), an original enzyme ATP hydrolytic enzymes. Third, determine the earliest use of oxygen family b.45.1.1 occur in approximately 29 million years ago, corresponding to the catalytic enzyme pyridoxal phosphate synthase (EC 1.4.3.5). Fourth, the evolution of metabolic network analysis, confirmed the principle of priority to add. Finally, by analysis the group transfer before and after the enzymatic reaction of the protein structure also contains the imprint of geochemical evolution. The research results show the great potential protein spatial structure can be used as molecular fossils \The protein structure as the molecular fossils inferred some molecular evolutionary event's provide important clues to explain the protein structure and function evolution and common biological problems. With the rapid development of genomics and structure of the genome, the method helps to find more useful information in the biochemical evolution.

Protein Architectures as Molecular Fossils to Trace Metabolic Evolution

Abstract

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