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Zinc Lipoprotein Gene At ZNF-1 Involved in Arsenate Reductase Gene AtACR2 Regulate Arsenate Resistance of Arabidopsis

Author: LiuAnNa
Tutor: BaiZuo；XuWenZhong
School: Inner Mongolia Agricultural University
Course: Genetics
Keywords: Arabidopsis Arsenic contamination The zinc fat gene Arsenate reductase Interaction
CLC: Q943.2
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
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Abstract


The metalloid arsenic exists in a variety of ores and minerals , mainly by skin contact , diet respiratory pathway into the human body , with the continuous development of modern industry , the content of arsenic in the environment is a steady increase in arsenic pollution has become a threat to human important issues of health and quality of life . Phytoremediation (phytoremediation) concept proposed by Brooks et al in the seventies of the last century , and in recent years has been developed as the primary means of arsenic pollution control . Phytoremediation Technology A plant to repair environmental pollution , and its objective is to develop a gene, to the the arsenic pollution control means are able to use in a plant of any species . Material , the use of the model plant Arabidopsis transgenic techniques to study the arsenate reductase gene (AtACR2) and reverse gene promoter - zinc lipoprotein (ZNF-1) interaction resistance to arsenic regulation . In wild-type Arabidopsis Columbia , T-DNA insertion mutation , the present experiments were body atacr2 -2 atacr2 -1 , the T-DNA insertion mutants znf-1 Arabidopsis as background material , with zinc lipoprotein gene agricultural the bacilli with dip flowers transformed import Arabidopsis , zinc grease genes in wild-type Arabidopsis Arabidopsis mutants atacr2-2, atacr2-1.zn-1 overexpression . Observed phenotype of different Arabidopsis plants identified arsenate reductase the gene (AtACR2) with zinc lipoprotein (ZNF-1) interaction can be increased to a certain extent the Arabidopsis resistance to arsenate , arsenate reductase gene in Arabidopsis study of the of arsenate stress response and regulation has an important role in this result greatly enrich the application of phytoremediation technology .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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