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The Role of GluCl Receptor in the Resistance of Plutella Xylostella (Lepidoptera: Plutellidae) to Abamectin

Author: LiuFeng
Tutor: WuQingJun
School: Chinese Academy of Agricultural Sciences
Course: Agricultural Entomology and Pest Control
Keywords: Plutlla xylostella abamectin nucleotide variance glutamate-gated chloride channel relative expression
CLC: S433.4
Type: Master's thesis
Year: 2011
Downloads: 83
Quote: 1
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Abstract


The diamondback moth, is a very important worldwide pest on cruciferous crops. Abamectin is an antibiotic insecticide with a high-efficiency and low-toxicity. However, because of using abamectin with an unreasonal way, a fast resistance to abamectin was emerged in the field populations of P. xylostella. GluCl receptor, being a major target of abamectin in P. xylostella, its role in abamectin resistance is unclear. The present study was trying to clarify the role of GluCl receptor in the resistance to abamectin through the indoor resistance selection, analyzing the potential mutation sites in the nucleotide sequence of different resistant strains and comparing the relative expression of GluCla.1. Laboratory selection of abamectin resistance in P. xylostellaThe original strain of P. xylostella was collected from the field of Fujian and reared in our laboratory for five years. Then it was selected by abamectin. Continuously selection of the strain was conducted for the last two years with a resistant ratio increasing from the initial 20.85-fold to the present 218.92-fold, which reached a high level of resistance. The resistance was increased slowly at the beginning but had a rapid elevation as more times of selection were conducted..2. Analyzing of the potential variance sites of the GluCla in abamectin resistant P. xylostella with different resistance levelThe GluCla was cloned from FT (180-fold resistance to abamectin), GT (30-fold resistance to abamectin), GH (217-fold resistance to abamectin), MD (513-fold resistance to abamectin), TH (680-fold resistance to abamectin), HN (534-fold resistance to abamectin) respectively. After blasting with all the copies of GluCla,16 positions of variances in the whole sequence were found in the nucleotide level and four of these variances resulted in the change of amino acids. Also, there are sequence insertion, deficiency, and institution in other sites. The four amino acids variances were only found in the GT and FT strains reared in the laboratory and not discovered in the populations collected in the field. All the other variances including substitution, deficiency and insertion existed in all resistant strains and populations with no significant difference among them.3. Analyzing of the relative expression of GluCla in abamectin resistant P. xylostella with different resistance level.Real-time Quantitative PCR was used to test the abundance of GluCla in the egg,1st instar,2nd instar, 3rd instar and 4th instar larval of FT and FM strains of P. xylostella. The result showed that in the instars tested in the experiment, the 1st instar of both FM and FT strains had the highest amount of mRNA expression but the 4th instar larval owned the biggest difference of mRNA expression that the abundance of GluCla in the FT was 7.47 times of which in the FM strain. At the same time, another test of the abundance of GluCla was conducted in the MD, TH, IVF and NK strains. The result proved that all these field populations shared a higher mRNA expression than the FM strain.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant pest and its control > Lepidoptera pests
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