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Research on Relationship Between Swainsonine and Endophyte of Undifilum oxytropis in Oxytropis Glabra DC.

Author: GaoXinLei
Tutor: HanBing；ZhaoMengLi
School: Inner Mongolia Agricultural University
Course: Biochemistry and Molecular Biology
Keywords: Oxytropis glabra Locoweed Swainsonine Endophytic fungi Undifilum oxytropis Quantitative PCR
CLC: S452
Type: Master's thesis
Year: 2011
Downloads: 71
Quote: 1
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Abstract


The genus Oxytropis (Oxytropis) perennial herb, Oxytropis glabra (Oxytropis glabra DC.) Legume (Leguminosae) is a locoweed northwest China grasslands . The locoweed (locoweed) leguminous perennial herb Astragalus (Astragalus) swainsonine (swainsonine, SW) is a certain amount of poisonous plants , livestock feed locoweed Oxytropis , showed symptoms of poisoning , serious cause livestock deaths , to the detriment of the development of animal husbandry . Locoweed toxin swainsonine and locoweed the endophytic fungus Undifilum oxytropis carry Studies have shown that , Oxytropis glabra as a member of locoweed also carry endophytic fungi . In this study, using quantitative PCR method to detect endogenous Oxytropis glabra the fungal Undifilum oxytropis with swainsonine relationship , the results are as follows: 1 detected in different organs from two populations of Oxytropis glabra swainsonine two populations florets swainsonine content in different organs of oxytropis there are differences , there is a significant difference ( P lt; 0.05 ) Yijinhuoluo County only of oxytropis glabra samples of roots and leaves swainsonine content . Isolated from Oxytropis glabra plant endophytic fungi , after homology analysis that the fungus belongs to Eric brick Alternaria endophytic fungi . In all from Oxytropis glabra different organs detected endophytic fungi , endophytic fungi content of the different organs of two populations of Oxytropis glabra , there are differences . The endophytic fungi content of the roots and leaves of the two populations of Oxytropis glabra individual there significant difference (P lt; 0.05); of Oxytropis glabra Yinjinhuoluo individual roots and stems within endophytic fungi content significant difference exists between ( P LT ; 0.05 ) . Four pairs of two populations of endophytic fungi content swainsonine content correlation analysis , Yinjinhuoluo Oxytropis glabra samples between students the fungal content with the appropriate content of swainsonine showed a significant positive correlation (r = 0.55, P lt; 0.05), between raw the fungal content with swainsonine content the Hangjinqi oxytropis glabra samples positive correlation ( r ≤ 0.1 , P gt ; 0.05) .

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CLC: > Agricultural Sciences > Plant Protection > Harmful plants and their removal > Poisonous weeds
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