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Objective: To investigate the impact of TNF-alpha human monocyte-macrophage cell line THP-1 TLR4 expression and function. Methods: (1) using different concentrations of TNF-a (10ng/ml, 20ng/ml, 50ng/ml, 100ng/ml) intervention THP-1 cells (24 hours), streaming technology to detect cell apoptosis, labeled cells of TLR4 expression, real-time PT-PCR detection of THP-1 cells TLR4 mRNA expression (2) experimental groups: TNF-a intervention group THP-1, TNF-a LPS intervention group, LPS intervention group and the control group ( No intervention factor group), after the intervention in each group was detected by Western blot inflammatory signaling pathway NF-B changes, detected by ELISA supernatant IL-6 content, extracted total RNA, real-time RT-PCR detection of different groups of cells IL-6, TNF-a mRNA expression levels change. Results: (1) flow results show that TNF-a was concentration dependent decrease in THP-1 cell surface expression of TLR4 above 50ng/ml reached a plateau, and the inhibition of apoptosis, Real-time RT- The PCR detection result is shown that TNF-a concentration dependent decrease in THP-1 cell surface expression TLR4mRNA above 50ng/ml reached the peak (P lt; 0.05). (2) Western blot test results are shown LPS alone stimulated THP-1 was significantly upregulated NF-kappa B protein levels, compared with TNF-a pre-stimulation of THP-1 after LPS stimulation, NF-kappa B protein levels were significantly decreased ( P lt; 0.05). Signaling pathway downstream expression of inflammatory factors: 1, the ELISA results are shown: LPS group, IL-6 content of the highest lower TNF-a LPS intervention group (P lt; 0.05): the control group and TNF-a separate intervention group IL-6 showed no significant differences. 2, Real-timePCR detection of IL-6, TNF-a mRNA expression results: LPS group, IL-6, TNF-a mRNA expression was significantly higher than TNF-a LPS intervention group (P lt; 0.05) Conclusion: TNF-a-induced THP-1 cell surface TLR4 expression decreased, and reduce its reactivity LPS. Objective: Adiponectin is secreted by fat cells endogenous insulin sensitivity factor, not only with increased insulin sensitivity, regulate glucose and lipid metabolism, and recent studies have found that the occurrence and development of the tumor cells also have some relevance, the paper analysis of gastrointestinal cancer patients with intra-abdominal adipose tissue adiponectin expression, and to explore its clinical significance. : Collection of clinical surgery 2008-2010, 39 cases of gastrointestinal cancer patients with intra-abdominal fat, grouped according to the results of the surgical biopsy, in which the transfer of the 21 cases, 18 cases without metastasis, non-tumor-bearing control group of 20 patients: pathological no tumor cells, not taking lipid-lowering hypoglycemic drugs, severe liver and kidney disease and the impact of endocrine and metabolic diseases in patients with intra-abdominal fat, and 20 cases; collect specimens were immediately placed in liquid nitrogen, total RNA extracted from adipose tissue reversed recorded for the cDNA using Real-Time PCR to detect the hormone adiponectin expression levels. Results: age, sex, general biochemical parameters among the three groups had no significant difference (p gt; 0.05), gastrointestinal cancer group adipose tissue adiponectin expression lower than control group, the difference was statistically significant (p lt; 0.05) , tumor metastasis group (3.85 ± 1.41) compared with non-metastatic group (4.9 ± 1.06) reduced adiponectin expression, but the difference was not statistically significant (p gt; 0.05). Conclusion: The expression of adipose tissue adiponectin new ways to reduce the incidence of gastrointestinal cancer, adiponectin may become cancer treatment, has important clinical significance.
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