Dissertation > Excellent graduate degree dissertation topics show

PPARγ expression and increased c-Ski Mechanism

Author: LiGongBo
Tutor: HeFengTian
School: Third Military Medical University
Course: Biochemistry and Molecular Biology
Keywords: Peroxisome proliferator-activated receptor γ c-Ski Atherosclerosis Vascular smooth muscle cells
CLC: R543.5
Type: Master's thesis
Year: 2011
Downloads: 49
Quote: 0
Read: Download Dissertation

Abstract


Background: Atherosclerosis (atherosclerosis, AS) serious harm to human health, it causes heart and cerebrovascular diseases to bring a physical and mental suffering, but also to society brought a heavy economic burden, research The molecular mechanism of the development of AS is the treatment of AS premise and key. In the development of AS many molecular mechanisms, peroxisome proliferator-activated receptor γ (peroxisome proliferators activated receptors, PPARγ) as a class of ligand-activated nuclear receptors, and its role has been fully confirmed. PPARγ can influence lipid balance and change the energy metabolism, regulation of inflammatory responses, and in the formation of foam cells and vascular smooth muscle cells (vascular smooth muscle cell, VSMC) proliferation and collagen secretion play a role, but the specific mechanism is not clear. The TGFβ / smad is another in the development of AS play an important role in the signaling pathway, which through smad pathway to promote VSMC proliferation and collagen secretion. c-Ski as a repressor of the signaling pathway can block the signal transduction pathway, the mechanism is smad3 the MH2 domain binding thereby preventing transcription complex formation and raise corepressor child. These studies suggested c-Ski as background TGFβ / smad pathway repressor of AS may play an important role in the occurrence and development, but on the c-Ski in the AS specific role and whether there is a correlation between PPARγ adjustable contact no report . Clarify this problem, you can clarify the interaction between the two in the AS and mechanisms for the treatment of AS to provide new ideas and strategies. Objective: To determine the AS PPARγ and c-Ski interconnected and influence of the AS. Methods: The rat through a high-fat diet AS model, testing AS plaque c-Ski mRNA and protein expression changes; in cultured rat vascular smooth muscle cells (vascular smooth muscle cell, VSMC) transfected with recombinant c-Ski plasmid and observe its effect on VSMC proliferation and collagen secretion in rats; using real-time PCR and Western blot PPARγ agonists and antagonists on the regulation of expression of c-Ski, and then use the online program NUBIScan and luciferase reporter Genetic testing for c-Ski clearly PPARγ regulation mechanism. Results: (1) AS rat arterial plaque c-Ski mRNA and protein expression was significantly reduced. (2) recombinant c-Ski after plasmid transfection significantly inhibited rat VSMC VSMC proliferation and collagen secretion. (3) PPARγ agonist rosiglitazone significantly increased rat c-Ski expression in VSMC, and this increase could be blocked by PPARγ antagonist GW9662. (4) bioinformatics analysis showed that the promoter region of c-Ski possible PPARγ binding sites, and rosiglitazone also significantly turned on dyed c-Ski promoter reporter gene expression. CONCLUSION: c-Ski effect with certain anti-AS, which may be another PPARγ target genes can activate PPARγ increases its anti c-Ski AS effect.

Related Dissertations

  1. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  2. The Changes of Intimal Macrophages, Smooth Muscle Cells, Collagen, and Cpt-Ⅰ, GLUT-4 of Vascular Media in Human Lower Limb Artery with Atherosclerotic Stenosis,R543.5
  3. Effect of High Glucose on Chemokine Ligand 16 (CXCL16) Expression and Secretion in THP-1 Macrophages and Intervention of Aspirin in the Activity,R587.2
  4. Correlation Research of Plasma Apolipoprotein CⅢ and High Sensitive C-reactive Protein with Atherothrombotic Cerebral Infarction,R743.33
  5. Role of G31P in Smooth Muscle Migration of A7r5 Cells Induced by Interleukin-8,R363
  6. The Effect of P-selectin on the Maturation of Dendritic Cells from Human Peripheral Blood,R543.5
  7. The Effects and Mechanism of Cellular Repressor of ELA-stimulated Genes on Pathological Vascular Endothelium Apoptosis,R363
  8. Carotid Artery Wall Elasticity and Carotid Atherosclerosis in Diabetes Mellitus Patients with Microalbuminuria Using Ultrasonographic Elastography and High-freguency Ultrasound,R445.1
  9. Correlative Study on Plasma Level of Resistin in Patients with Coronary Atherosclerosis Disease,R541.4
  10. Experimental Study on Antiatherosclerosis with EL Antisense,R543.5
  11. Effects of Receptor Activity Modifying Protein-1 Transplantation of Mesenchymal Stem Cells on Postangioplasty Restenosis and Cardiac Function after Myocardial Infarction in Rabbits,R542.22
  12. Relationship Between Chronic Obstructive Pulmonary Disease and Coronary Atherosclerotic,R563.9
  13. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  14. Shanxi Province, the development of ski tourism Investigation and Research,F592.7
  15. Heart rate variability and coronary artery disease and prognosis of acute myocardial infarction,R541.4
  16. 3-year changes in lipopolysaccharide newly diagnosed type 2 diabetes and atherosclerosis in patients with the correlation between the evolution of research,R543.5
  17. Ischemic stroke and carotid atherosclerosis and related biochemical hs-CRP and LDL-C changes,R743.3
  18. Migration of VSMC and SPC by Expression of Sdf-1αin VSMC on the Effect of ox-LDL,R363
  19. The Homestasis of Regulatory T Cells in Patients with Acute Coronary Syndrome,R541.4
  20. Anti-inflammatory effect of fusion protein anticoagulant dual function of TAP-SSL5,R543.5
  21. The Effects of Rosiglitazone and Prednisone on the Expression of FIZZ1 in the Pulmonary Fibrosis Induced by Blemycin,R965

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Vascular disease > Artery disease
© 2012 www.DissertationTopic.Net  Mobile