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The Interference Effect of Procyanidins on Semicarbazide-Induced Immune Injury in Mice

Author: YuMingDong
Tutor: LiDongYang
School: Nanhua University
Course: Health Toxicology
Keywords: Proanthocyanidins Semicarbazide KM mice Immune injury Antioxidant
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 46
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Abstract

Objective 1 was observed procyanidins (proanthocyanidins, PC) on semicarbazide (semicarbazide, SEM) induced immune injury intervention effects, and compare immune damage before and after adding proanthocyanidins intervention; 2 explore proanthocyanidins on semicarbazide in mice the possible protective effect of immune injury mechanism for the further development and utilization of proanthocyanidins provide a theoretical basis. Methods 80 clean adult Kunming (KM) mice, male and female, were randomly divided into 8 groups of 10: solvent control group, SEM exposure group, SEM after exposure plus low, medium and high dose of PC protection group, low, medium and high doses PC protection, add SEM exposure group. Solvent control group fed with deionized water; SEM exposure group at 56.25 mg / kg · bw SEM solution gavage for 2 weeks, then continue with deionized water orally four weeks; SEM after exposure plus low, medium, PC protection high dose group, give three groups of animals are carried 56.25 mg / kg · bw SEM solution gavage two weeks, and then according to the design of PC were protective dose (100,200,400 mg / kg · bw) to continue filling Stomach four weeks; low, medium and high doses PC protection, add SEM exposure group, the first three groups of animals were given according to the design of PC protective dose (100,200,400 mg / kg · bw) orally for 4 weeks, respectively, and then continue at 56.25 mg / kg · bw SEM solution gavage for 2 weeks. These solutions were each group of animals fed with deionized water as solvent, according to the volume 10ml/kg · bw orally, intragastrically 6 weeks, 1 day. 24h after the last administration, eyeball blood, serum preparation kit serum SOD activity and MDA; mice were sacrificed by cervical dislocation, neutral red assay mouse peritoneal macrophage phagocytosis; sterile preparation spleen spleen cell suspension, modified MTT assay NK cell activity; hemolytic plaque assay antibody-forming cells; MTT determination of T-cell proliferation. Cut the mouse left and right ears and ear piece 8mm in diameter were collected and weighed to calculate the degree of swelling of mice was measured delayed type hypersensitivity (DTH); separating each group of mice thymus, spleen, weighed and calculated organ coefficient; conventional pathology sections, HE staining of spleen tissue morphology. Results 1, SEM body weight of mice exposed to growth is lower than the solvent control group (P lt; 0.05); low, medium and high dose groups of mice protected PC first, respectively, higher than the PC after weight gain protection group, high dose group PC difference was statistically significant (P lt; 0.05). 2, SEM exposure group spleen, thymus organ coefficient decreased compared with the control group (P lt; 0.05); in PC protection after high-dose group of mice spleen coefficients higher than SEM exposure group (P lt ; 0.05); low, medium and high doses PC protection group first thymus organ coefficients were higher than after the same dose of PC protection group, the difference was statistically significant (P lt; 0.05), and a dose - response relationship. 3, spleen histopathology Show: solvent control group spleen capsule intact smooth, splenic corpuscle structural integrity, red, white pulp clear boundaries, the number of normal red pulp of the spleen index, spleen normal sinus arrangement, white pulp germinal centers clear arteries surrounding normal lymphocytes; SEM exposure group splenic corpuscle structural damage, red, white pulp unclear boundaries, reduce the number of splenic cord, spleen sinus disorder, germinal center and marginal zone blur, reduce arterial peripheral lymphocytes; PC protection group after spleen morphology exposure group improved compared with SEM, and with the PC more obvious improvements to increase the dose, splenic corpuscle institutions increasingly full, red marrow boundaries increasingly clear increase in the number of germinal centers increasingly obvious, arterial gradual increase in the number of peripheral lymphocytes; PC first protection group structure spleen splenic germinal centers and the number of lymphocytes around the artery to improve protection after PC group compared the effect is more obvious. 4, compared with the control group, SEM NK cell activity in mice treated group decreased (P lt; 0.05); exposure group compared with the SEM, medium and high doses of PC protection group after the mouse NK cell activity increased, difference was statistically significant (P lt; 0.05); medium and high dose groups of mice protected PC first activity of NK cells with the same dose of PC protection group after all increased (P lt; 0.05), and a dose - effect relationship. 5, SEM infected mice peritoneal macrophage phagocytosis of neutral red with the solvent control group decreased (P lt; 0.05); medium and high doses of PC protection group after peritoneal macrophages in phagocytosis of red exposure group compared with the SEM has increased, the difference was statistically significant (P lt; 0.05); compared with the same dose of PC, PC and PC protection group after the first group of mice peritoneal macrophages Protection neutral red cell phagocytosis was no significant difference (P gt; 0.05). 6, SEM exposure group compared with the control group, reducing the number of mouse antibody-forming cells (P lt; 0.05); in PC protection after high-dose group compared with the SEM exposed mice increased the number of antibody-forming cells ( P lt; 0.05); medium and high doses of PC protection group with the same dose of the first post-PC protection group can increase the number of mouse antibody-forming cells (P lt; 0.05). 7, compared with the control group, SEM exposure groups reduced lymphocyte proliferation force (P lt; 0.05); medium and high doses of PC protection group after exposure group compared with the SEM, lymphocyte proliferation force increased, the difference was statistically significant (P lt; 0.05); medium and high dose PC protection group first lymphocyte proliferation power than the same dose of PC protection group were significantly increased after (P lt; 0.05). 8, SEM exposure group compared with the control group of mice can reduce swelling around the ear piece (P lt; 0.05); PC protection group after exposure group compared with the SEM left mouse ear swelling degree increased (P lt ; 0.05); PC first group of mice protected swelling around the ear piece and after the same dose of PC protection groups were improved compared, the difference was statistically significant (P lt; 0.05). 9, compared with the control group, SEM exposed mice compensatory increase in serum SOD activity, MDA content increased (P lt; 0.05); medium and high doses of PC protection group after exposure group compared with the SEM , serum SOD activity increased (P lt; 0.05), MDA content decreased (P lt; 0.05); medium and high doses of PC protection group first serum SOD activity and after the same dose of PC protection groups were compared was increased (P lt; 0.05), MDA levels were significantly lower (P lt; 0.05), and showed a dose - response relationship. Conclusion 1 dose (200-400mg/kg · bw) proanthocyanidins (PC) for the protection or mitigation semicarbazide (SEM) in mice caused by immune injury; 2, immune injury after injury before adding PC than the effect of adding more PC obvious; 3, PC on SEM induced immune injury mechanism may be oxidative damage.

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