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As lifespan expanding and the aging of the population becoming serious, osteoporosis （OP） has become a common disease and serious harm to human health.Meanwhile, the incidence and mortality of Cardiovascular disease（CVD） has outstriped tumors which makes it one of the most serious disease in the world. Ligusticum chuanxiong Hort.and Salvia miltiorrhiza Bge,both are versatile and abundant resources of traditional chinese medicine,have been used in the treatment of numerous ailments. Many drawbacks in traditional extraction techniques had hindered the further application of natural active ingredient, such as low efficiency, pollution and organic solvent residual. In this dissertation, ultrasound enhanced supercritical CO2 extraction （USCE） technique was introduce to extract effective ingredients from compound recipe of Ligusticum chuanxiong Hort and Salvia miltiorrhiza Bge.The yield of the extract, tetramethylpyrazine ,ferulic acid and tanshinone IIA were determined to obtain the optimal processing parameters of supercritical CO2 extraction （SCE） and ultrasound enhanced supercritical CO2 extraction（USCE）.The efficacy of active ingredients on osteoporosis model were also studied.By means of single factor experiments and orthogonal design experiments, the optimal processing parameters of SCE were : extraction pressure 25MPa ,extraction temperature 55℃, the meterial particle size 60 mesh,cosolvent amount 1.0mL/g ethanol.Under these conditions,the yield of the extract was 2.84% （RSD=1.53%,n=3） after 2h extraction,the yield of tetramethylpyrazine ,ferulic acid and tanshinone IIA were: 125.74μg/g（RSD=0.84%,n=3）, 367.66μg/g （RSD=1.23%,n=3） and 131.31μg/g （RSD=0.81%,n=3） respectively.The significance of the four major factors affecting effective yield of USCE was in the following order: extraction pressure>extraction time >extraction temperature>ultrasound power density.The optimum extraction combination were: extraction pressure 19MPa ,extraction temperature35℃, ultrasound power density 120W/L, extraction time 1h. Under these conditions,the yield of the extract was 3.85% （RSD=1.76%,n=3）,the yield of tetramethylpyrazine ,ferulic acid and tanshinone IIA were: 150.64μg/g （RSD=0.99%,n=3）, 425.33μg/g （RSD=2.09%,n=3） and 203.65μg/g（RSD=2.36%,n=3） respectively.The results of different extract method extracting effective components from compound recipe of Ligusticum chuanxiong Hort.and Salvia miltiorrhiza Bge indicated that: USCE was superior to both SCE and organic solvent leaching ectraction. The dynamic equation of model predictions of SCE and USCE are: Y = 3.10938×(1-e-0.76942×t) and Y = 3.89091×(1-e-1.32226×t).Simultaneous extraction was superior to individual extraction which reflected in the yield of the effective components and the scanning electron microscopy（SEM） of the differently processed meterial.The results of the efficacy of Ligusticum chuanxiong Hort USCE extract（A）, Salvia miltiorrhiza Bge USCE extract（B） and the simultaneous USCE extract（C） on osteoporosis model demonstrated that extract species,extract patten and extract concertration level had influence on percentage of OD value of UMR106cells.At level of 0.1μg /mL,A,B,C had similar efficacy,the promotion on the proliferation of UMR 106 cells were tardily; at level of 1.0μg /mL, the efficacy of A,B,C had reached the remarkable level compared to normal control;at level of 10.0μg /mL,C had more obvious effect,and reached the extremely remarkable level. at level of 100.0μg /mL,A and B showed inhibition on cells proliferation,but C also can subserve cells proliferation.At level of 10μg /mL, B and C all showed the stimulative effect. At level of 100μg /mL,all three USCE extracts showed the cell differentiation promotion activities,the statistics were extremely significant differences.
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