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Rheumatoid arthritis (rheumatoid arthritis, RA) is one of the worldwide refractory major diseases with high morbidity , serious threat to human health . To date , clinical still a lack of effective treatment options to control the progression of the disease , preventive measures and treatment drugs . In Chinese medicine, classified as paralysis disease areas , acupuncture for RA significant clinical effect , has become an effective way of TCM clinical treatment of paralysis disease and an important means of treatment . But more for the rheumatoid arthritis studies focused on the clinical aspects of the mechanism of acupuncture to improve rheumatoid arthritis less . Based on the above considerations , we designed the experiment to investigate the impact of acupuncture for the AA rat model of immune cells . We complete Freund's adjuvant -induced SD rats as a model of rheumatoid arthritis ( AA model) , select the full three years as the point of stimulation , the AA rat model of electrical stimulation to observe the different groups of rats arthritis index , ankle swelling , peripheral blood T cell subsets , intestinal PP knot number and Tregs cells and immune factors change , and to explore the mechanism of acupuncture treatment of RA . 30 male SD rats were randomly divided into three groups: normal group , model group , electro-acupuncture group . Model group , acupuncture group in the rat foot pads at the injection complete Freund adjuvant 0.1ml / only from the eighth day of the modeling every 72 hours for electrical stimulation of the acupuncture group , and to evaluate the rats joint inflammation index , ankle swelling , stimulation were sacrificed 13 times . HE staining observed under light microscope ankle pathological damage ; flow cytometry in whole blood CD4 , CD8 T cells and the number of PP junction Tregs cells ; ELISA ELISA in serum TNF - a , IL - 1 , IL - 10 content . Conclusion: 1 EA Zusanli symptoms of AA rat model has improved , and the acute phase is more obvious . The power Tsusanli AA rat model of peripheral blood CD4 D25 Tregs cells has a regulatory role . 3 power Zusanli to increase in the content of the AA rat model of chronic peripheral blood CD8 T cells , to reduce CD4 / CD8 ratio . 4 EA Zusanli intestinal mucosal immune ( pp knot ) and pp junction CD4 CD25 Tregs had no effect . 5 EA Zusanli can reduce TNF - alpha , IL - 1 content , increased IL-10 content .
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