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Effects of Carbon Monoxide on Vase Life of Cut Rose Flower and Salt-Induced Programmed Cell Death in Wheat Primary Root

Author: LingTengFang
Tutor: ShenWenZuo
School: Nanjing Agricultural College
Course: Biochemistry and Molecular Biology
Keywords: Carbon monoxide Hemin Cut Roses Vase life Wheat primary root Programmed cell death
CLC: Q945
Type: Master's thesis
Year: 2008
Downloads: 124
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Abstract

Carbon monoxide (Carbon monoxide, CO) is a diatomic gaseous molecules. Over the years, as atmospheric pollution generated CO has been considered is a toxic gas. Large number of studies recently confirmed that CO is the messenger between cells of animals involved in the regulation of animals many important physiological and pathological processes. CO synthesis also the main source of heme oxygenase (Heme oxygenase, HO) has been found in Arabidopsis, and encoding the HO gene has been cloned and expression, and was confirmed in vitro can catalyze CO. The other teams in our laboratory have found that CO plays an important role in the regulation of normal plant development and stress, including the regulation of stomatal closure, promote the occurrence of mung bean adventitious roots and rapeseed lateral roots, and can increase the biomass of rice under salt stress. To further study the CO whether plant senescence and programmed cell death (Programmed cell death, PCD) in the starting role, the experiments were cut roses and wheat primary root for the materials studied, the main research content and results are as follows: exogenous CO donor hematin an exogenous CO donor cut rose vase life and anti-oxidative metabolism of different concentrations (0.001, 0.01, and 0.11μmol · L ^{-1 ) (Hematin, H) on the vase life of cut roses 'movie star' and anti-oxidative metabolism. The results show that, compared with the control (CK), CO donor hematin of 0.001-0.1μmol, L ^{-1 use within extend the vase life of cut rose with the increase in the concentration, which 0.01μmol · L ^{-1 of hemin (H0.01) significant role in preservation of cut flowers, vase life than CK can extend 57.4%; 0.1μmol · L ^{-1 hemin but shorten the vase life of cut rose by 13.1%. Further studies showed that, the H0.01 deal with different degrees of increase early POD, CAT and SOD activity, and thereby significantly reduce the lipid peroxidation. 2 CO under salt stress of wheat primary root PCD different concentrations of NaCl (100 mmol · L ^{-1 -400 mmol · L ^{-1 ) showing primary roots of wheat The concentration-dependent growth inhibition, accompanied HO activity decreased. We choose moderate stress salt concentration (200mmol · L ^{-1 NaCl) study found that adding different saturation (5%, 50% and 100%) CO aqueous solution can significantly alleviate salt stress on wheat primary root inhibition effect, delay the occurrence of PCD, including the lowering of the primary roots of the extent of the DNA ladder and TUNEL staining positive nuclei proportion, the most obvious effects of a saturated aqueous solution of 50% CO, 24 hours of primary root growth rate than salt stress 13.42%, and six hours of TUNEL-positive nucleus decline in the proportion of 26.36%. The same nuclear chromatin evidence 50% CO saturated aqueous solution treatment can reduce the cohesion of the growing point near the nucleus and marginalized. These phenomena show that CO might have to slow root growth inhibition of salt on wheat newborn PCD by delaying the primary roots of wheat under salt stress. Further study confirmed that CO is likely by upregulating the SOD (Mn-SOD and Cu / Zn-SOD) gene expression and downregulation of NADPH oxidase gene expression, leading to the root of the superoxide anion decreased, to reduce the toxic effects of salt ions. Specific application superoxide anion scavenger Tiron NADPH oxidase inhibitor Apopcin and the Cu / Zn-SOD inhibitor DETC 50% CO saturated aqueous processing and display, the former two primary roots of wheat under salt stress suppression can reduce effect, but to ease the effect is less than 50% CO saturated aqueous solution. While DETC effective reversal of the effects of 50% CO treatment, suggesting that CO ways to promote not only by adjusting the superoxide anion wheat root growth under salt stress, but also there may be other ways.}}}}}}}

Effects of Carbon Monoxide on Vase Life of Cut Rose Flower and Salt-Induced Programmed Cell Death in Wheat Primary Root

Abstract

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