Dissertation > Excellent graduate degree dissertation topics show

Primary Study on Polymerized Bovine Hemoglobin Using Sulphydryl-cross-linkers

Author: MaZuo
Tutor: WangJinFeng
School: Tianjin University
Course: Medicinal Chemistry
Keywords: Oxygen carriers Polymerized bovine hemoglobin Sulphydryl- cross-linkers Molecular weight distribution
CLC: R91
Type: Master's thesis
Year: 2008
Downloads: 108
Quote: 0
Read: Download Dissertation

Abstract


This thesis focuses on polymerized hemoglobin as a kind of oxygen carriers. Oxygen carriers could be widely used. They can not only apply as blood substitutes, but also use as oxygen therapeutics. Polymerized hemoglobin has been studied for about twenty years. However, it failed to approve clinical trial phase III because of its two main flaws. One is that its complex composition makes its property very uncontrollable. The other is that the product contains a little of unpolymerized hemoglobin which will cause the product very unsafe.My proposal is to solve these two main flaws. I use sulphydryl-cross-linkers such as dimaleimides instead of amino-cross-linkers such as glutaraldehyde to polymerize hemoglobin. This proposal would solve the problems. First, sulphydryl groups (about two) are much less than amine groups (more than forty) on the surface of hemoglobin. Therefore, the process of polymerizing is much easier and more controlable. Sencond, the reaction has a very high activity and is almost quantitative yield. So it will decrease the ratio of unploymerized hemoglobin.By electrophoresis, size exclusion chromatography and other analyses, my work has proved that under pH 9 condition, the product using sulphydryl-cross-linkers such as dimaleimides has easier composition than the product using glutaraldehyde does.

Related Dissertations

  1. Study on Removal Characteristics of Dissolved Organic Matters from Bio-treated Effluent of Dye Wastewater by Activited Carbon Adsorption,X791
  2. Study on the Reaction Performance of Iron- and Nickel-based Oxygen Carrier for Chemical Looping Combustion,TK16
  3. Pine nut pine antioxidant peptides and anti- tumor peptide preparation and functional identification of,R285
  4. Lyophilization of Hemoglobin-based Oxygen Carrier and Storage of the Freeze-Dried Solids,Q816
  5. Study of Selective and Quantitative Measurement of Hemoglobin and/or Related Derivatives in the Body Fluid,R96
  6. Study of Glutaraldehyde-polymerized Porcine Hemoglobin as Hemoglobin-based Oxygen Carrier,TQ460.1
  7. Application of Biochip Methodology in Studies of Specific Antigenecity and Quantitative Assay of Hemoglobin,Q51-3
  8. A Comparative Study of the people , cattle , pig hemoglobin and glutaraldehyde-polymerized epitopes,Q51
  9. Basic Study on Partial Oxidation of Methane to Syngas Use Lattice Oxygen,TE665.3
  10. Effect of Mixed Liquor Characteristics on Membrane Fouling in Membrane Bioreactor,X703
  11. Research on Combustion Synthesis of Fe2O3-based Oxygen Carrier and Its Performance Used in Chemical Looping Combustion,TK16
  12. Study on the Application of Coagulation-microfiltration Process for Treatment of Micro-polluted Water and Low-radioactive Wastewater,X703
  13. Dynamic Optimization of Molecular Weight Distribution for Batch Free Radical Polymerization,O631.3
  14. Study on Technologies of Mitigating Membrane Fouling in Membrane Bioreactors,X703
  15. Inorganic and inorganic - organic hybrid Zn -based catalysts and catalytic L- lactide ring-opening polymerization of L- polylactic acid applied research,O631.3
  16. Synthesis, Characterization and Crystallization Behavior of Isotactic Polystyrene and Ethylene-styrene Copolymer,TQ316.3
  17. Study on Polymer Reological Behavior and Structure-properties Prepared Via Low Frequency Vibration Injection Molding,TQ320.5
  18. Functionality and Nutritional Properties of Foxtall Millet Protein and Its Hydrolysates Mohamed Tabita Kamara,TS210.1
  19. Study on Methods for Membrane Fouling Control in Membrane Bioreactor,X703.1
  20. Study on the Molecular Characterization, Rheological Properties and Physical Cryogel of Mucopolysaccharide Hyaluronan,O636.1

CLC: > Medicine, health > Pharmacy > Drug basic science
© 2012 www.DissertationTopic.Net  Mobile