Dissertation > Excellent graduate degree dissertation topics show

Immunoregulatory Effects of Thalidomide on Systemic Lupus Erythematosus (SLE) and Rheumatoid Arthritis

Author: YinZuoYing
Tutor: LiJing
School: Jiangsu University
Course: Internal Medicine
Keywords: thalidomide systemic lupus erythematosus fibroblast-like synoviocytes rheumatoid arthritis IL-17
CLC: R593.241
Type: Master's thesis
Year: 2009
Downloads: 40
Quote: 0
Read: Download Dissertation


Objective:To detect the immunoregulatory effect of thalidomide on peripheral blood T-lymphocytes of systemic lupus erythematosus patients,Fibroblast-like synoviocytes of SD rats and Thl7 cells of RA patients.Methods:The experiment is divided into three parts(1)Part one:the experimental part with SLE patients:isolated the mononuclear cells from the peripheral blood of SLE patients,incubate the cells to 24 hole cell culture flask.The cells were kept under the conditions of 37℃,5%CO2 for 2h.Then discard the adherent cells,collected cell suspension.Re-suspended the cells with DMEM that contained 10%FBS.Sub-groups: the control group,DMSO group,according to the concentration,Thd groups are divided into five:2.5、25、100、300、500μg/ml,after 72h’s incubation these T cells’ proliferation was deteted by MTT while after 24h’s incubation the apoptosis、CD28 expression by CD3+T lymphocyte and CD152 expression by CD8+T lymphocyte were analyzed by flow cytometry(FCM).The mRNA expression of IL-6、IL-10 and TNF-αwere measured by RT-PCR after 72h.(2) Part two:the experimental part with Fibroblast-like synoviocytes of SD rats.Selection of normal SD rats,the fibroblast-like synoviocytes(FLS) are separated and cultured by collagenase and trypsin digestion way.When the cells were subcultured to the third generation,thalidomide was added and stayed for 24 hours. Sub-groups:the control group,DMSO group,according to the concentration,Thd groups are divided into five:300、500、800μg/ml.The proliferation was deteted by MTT while apoptosis were analyzed by flow cytometry.(3)Part three:the experimental part with RA patients.sub-group:accordance with the disease activity score(DAS) the patients are divided into three groups:RA disease activity group,RA stability group and the healthy control group.Mononuclear cells were separated from the peripheral blood of the RA patients.CD4+T cells which were negatively selected by immunomagnetic beads will be stimulated by PMA/Ion.At the same time an intervention of thalidomide(final concentration:500μg/ml) will be kept in the system for 6 hours.After the next steps of fixing,anti-reflective coating and intracellular staining,the level of IL-17 will be detected by FCM.Results:(1) In vitro 5001ag/ml thalidomide inhibited the expression of CD3+CD28,however,it promoted the early apoptosis;100μg/ml~500μg/ml thalidomide inhibited the proliferation,while promoted the expression of CD8+CD152.500μg/ml thalidomide inhibited the mRNA expression of IL-6,2.5μg/ml~500μg/ml thalidomide inhibited IL-10、TNF-αmRNA expression.(2) Thalidomide can inhibited the proliferation of FLS.It can also induce the apoptosis of FLS.Both these effects are concentration-dependent.(3) after the intervention of Thd,the intracellular expression of IL-17 by CD4+T cells from the peripheral blood of RA disease activity group,RA stability group and the healthy control group are obviously decreased,the difference was statistically significant.Conclusions:Thd have shown immunosuppressive effects on peripheral blood T lymphocytes of SLE patients,Fibroblast-like synoviocytes of SD rats and the intracellular expression of IL-17 by CD4+T cells from the peripheral blood of RA patients.It may reverse the immune imbalance of patients with autoimmune disease,making it possible to achieve therapeutic purposes.

Related Dissertations

  1. Blockage type of rheumatoid arthritis clinical research for Zhou Naiyu academic thinking and clinical experience and Bi Yu Kang I,Treatment of late alpine,R259
  2. Design, Synthesis and Antitumor Activity of thalidomide derivatives,R965
  3. Effects of Blockade of IL-17 in Already Established Asthma of BALB/c Mice,R562.25
  4. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  5. Clinical Analysis of 33 Cases of Pediatric Systemic Lupus Erythematosus,R725.9
  6. OX40/OX40L peripheral blood lymphocytes in patients with SLE characteristics and biological significance of the expression,R593.241
  7. The Expression and Significance of Serum MIP-1β and MCP-1in Patients with Rheumatoid Athritis,R593.22
  8. Effect of Thalidomide on Cellular Growth and Angiogenesis of Human Pancreatic Carcinoma Cell Line SW1990,R735.9
  9. Genetic Variation in CD226 Gene and Systemic Lupus Erythematosus,R593.241
  10. Experimental Study of Th17 Related Factors of Animal Models Infected with Mycoplasma Suis,S858.28
  11. Effects of Hyaluronic Acid on Osteopontin and CD44 of Fibroblast-like Synoviocytes in Patients with Osteoarthritis of the Knee,R684.3
  12. Diagnostic Value of Ultrasound for Imaging of MCP, PIP and Wrist Joints in Rheumatoid Arthritis-a Comparative Study with Magnetic Resonance Imagin,R593.22
  13. Aplication and Development of Cystatin C Kit,R593.241
  14. The Study of Levels of Toll-like Receptor 9 and IL-10、 IFN-α in Patients with Systemic Lupus Erythematosus,R593.241
  15. The application of the theory of collateral disease in systemic lupus erythematosus,R259
  16. Gui Shao Tong Luo Tang mixed cold and heat treatment of rheumatoid arthritis clinical research evidence,R259
  17. The Correlation Reasearch between Pharmacokinetic of Methotrexate Treatment RA and Clinic Effect,R96
  18. Analysis of Th17 Cells and Regulatory T Cells in Myasthenia Gravis,R746.1
  19. TP on the AIA rat VEGF, FLT1 and CCR5 expression,R684
  20. Effects of Chinese Herb Clearing Heat and Removing Dampness Combining with Western Medicine on Rheumatoid Arthritis Patients in Active Phase: a Systematic Review,R593.22
  21. HIF-1α, VEGF in type Ⅱ collagen -induced arthritis model of Expression and significance,R593.22

CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Lupus erythematosus > Systemic lupus erythematosus
© 2012 www.DissertationTopic.Net  Mobile