Dissertation > Excellent graduate degree dissertation topics show
Production and Preliminary Application of a Visual Protein Chip to Differentiate Antibodies of Four Poultry Diseases
Author: ShiLin
Tutor: WangXiuRong
School: Chinese Academy of Agricultural Sciences
Course: Preventive Veterinary Medicine
Keywords: protein chip avian diseases antibody diagnose
CLC: S852.65
Type: Master's thesis
Year: 2009
Downloads: 152
Quote: 0
Read: Download Dissertation
Abstract
|
Biochip technology, which was developed along with the human genome project (HCG), is a new technique and method applied in life science research. As the gene chip technology has gradually become mature and many outstanding achievements have been obtained, the use of protein chip technology emerged relatively recently. Protein chip technology has shown its great potential in the functional analysis of the proteome, disease diagnosis and drug development.To produce a visual protein chip that can detect antibodies against avian influenza virus, newcastle disease virus, infectious bronchitis virus and infectious bursal disease virus simultaneously. The establishment of protein chip include the selection of suitable substrate, the delivery of capture molecular, the hybridization of antibody and the removal of unspecific binding.Purify the 4 viruses protein with Ultracentrifugation and sucrose density gradient centrifugalization method ,the proper concentration purified protein were spotted on a aldehyde group modified glass slides respectively to generate protein chips. Positive serum against these viruses were hybridized to these antigen, then the bound antibodies were detected with nano-gold labelled second antibody and developed with silver staining method, a platform of a visual protein chip to differentiate 4 kinds of avian diseases was constructed.; Furthermore, we have studied specificity and the correlation of intensity valuabel with the concentration of antibodies of this visual protein chip, then the visual protein chip was used to test 18 specimen .After the condition was optimized, relative antigen could be hybridized specifically with the probe of this protein chip and strong signal was showed. Intensity valuabel showed linear reference in the concentration range of 50μg/mL～300μg/mL of antibodies. This protein chip showed good sensibility compared with traditional method. Through a series of optimization experiments, an optical protein chip for differentiating antibodies of four poultry diseases was constructed. This chip is characterized by rapidity, simplicity, high sensitivity, good specificity, no need for special instrumentation and low cost. The detection reliability of serum specimens is high and the sensitivity is also higher than that of the agar diffusion reaction, and it could become a popular application.This method, which combines the immune colloidal gold tech(?)ique, the silver developing technique and protein chip technique, selects purified totivirus protein as the captured antigen for the first time, and is an innovation and breakthrough in serum detection techniques. Our visual protein chip will play a considerable role in promoting the popularization of protein chip technology; this technology lays a good foundation for further construction of integrated chips of high density for detecting multiple antibodies.
|
Related Dissertations
- Study on Bispecific Monoclonal Antibody to Pesticide Imidacloprid and Parathion-Methyl,S482.2
- Establishment of ELISA for Detecting Cd-MT of Pteria Penguin and Preliminary Study of ELISA-kit,X835
- Expression and Purification of Influenza a Virus M2 Protein and Detection of Its Antigenicity,R392
- Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
- Studies on Dynamic Regularity of Antibody Against G. Anatis and Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against G. Anatis Lps,S858.32
- Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against Lps Antigen of B. Melitensis,R392
- The Research on Enzyme-Linked Immunosorbent Assay for Pretilachlor and Acetochlor,S482.4
- Epidemiologic Studies on Duck Circovirus Infection in East China and Development of Monoclonal Antibody Against Duck Circovirus,S858.32
- Establishment of ELISA Based on Recombinant Protein NS1 of Swine Influenza Virus and Nuclear Localization of SIV NS and NP Proteins,S858.28
- A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
- Purification of Recombinant Schistosoma Japonicum Protein SjGST and Preparation and Characterization of the Monoclonal Antibody,R392
- Research of Electrochemical Immunosensor for Schistosomiasis Japonicum Diagnosis,R532.21
- The Influence of Early Intervention of Vitamina Combined with Mycobacterium Bovis Bacillus Calmette-Guerin on Lung CD11C+ and Anti-OVA Special IgE Expression in Adult SD Mice Asthma Model,R562.25
- Cloning, Identification and Eukaryotic Expression of Variable Region of Monoclonal Antibodies Against Chelated Mercury, Copper and Zinc and Three Dimentional Modeling of Recombinant Antibody,X171.5
- Serological Supervision and Prevention of Porcine Reproductive and Respiratory Syndrome in Large-scale Swine Farm,S858.28
- Clinical Significance of the Anti-erythropoietin Antibody in Maintenance Dialysis Patients,R692.5
- Efficacy Evaluation of Egg Yolk Immonoglobulins (IgYs) Against Solobacterium Moorei Causing Halitosis,R392.1
- Studies on the Interaction between Biomolecules by Surface Plasmon Resonance Technology and Fluorescence Spectrometry,Q503
- The Study on the Preparation of McAb to Anti-Melemine and Establishment of Ic-ELISA for Detetion of Melamine,S859.84
- Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
- Study of the Theraputic Effect of Anti-human B7-1 Antibody on Lupus-like Disease,R593.242
CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Livestock Virology
© 2012 www.DissertationTopic.Net Mobile
|