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Objective: ① To determine PROM patients and normal term pregnancy fetal membrane tissue MPO, LPO, SOD, GSH expression levels of oxidative damage and premature rupture of membranes. ② investigate antioxidant oxidation in the pathogenesis of premature rupture of the role. Methods: a study: (1) PPROM group: Select October 2007 - Jun 2008 Second Hospital of Hebei Medical University hospital delivery check and 20 patients with premature rupture of membranes , occurred in the 37 weeks before called PPROM, premature rupture of membranes diagnostic criterion-referenced 6 edition of \1-36 weeks, mean 35 ± 0.75 weeks. (2) PPROM group: Select year term premature rupture of membranes occurred in 20 cases, occurred in the 37 weeks after called premature rupture of membranes, the group of pregnant women aged 26-35 years, mean 29.1 ± 3.21 years of age, gestational age 386-403 weeks, mean 39.2 ± 0.63 weeks. (3) normal pregnancy group: Select the same period in our hospital 20 cases of normal pregnant women giving birth, age 25-30 years, mean 27.67 ± 1.86 years of age, gestational age 385-41 weeks, mean 39.33 ± 1.21 weeks. All pregnant women are single cephalic, no pregnancy complications and comorbidities. 2 Specimen collection: at vaginal delivery or cesarean section after delivery of the placenta and the blood on the surface speed to remove amniotic fluid specimens from nearly two membranes break at 1.5cm × 1.5cm size, set-70oC refrigerator spare. Subjects for each one name, age, body mass index, occupation, motherhood, gestational age, mode of delivery and cesarean section because, rupture of fetal membranes collected specimens to time etc detailed records of each specimen are clearly marked after storage. 3 Experimental Methods: After collecting specimens homogenized tissue, according to kit instructions, MPO activity was measured using colorimetric assay; LPO thiobarbituric acid (TBA) assay; SOD activity assay with xanthine, xanthine oxidase reaction system to Chemiluminescence determination; GSH expression levels were measured by colorimetry. 4 Statistical analysis: All data were processed using SPSS13.0, the results are expressed as mean ± standard deviation (? X ± s) said, using t-test, ANOVA, correlation analysis using Pearson correlation analysis to α = 0.05 As a test of significance level. Results: 1 SOD in PROM group and normal pregnancy group compared expression levels: SOD in normal pregnancy group average is 308.43 ± 22.54 U / mgprot, in PROM group is the average of 248.34 ± 22.23 U / mgprot compared with normal pregnancy group difference was statistically significant (P lt; 0.05). 2 SOD in PPROM group and PPROM group compared expression levels: SOD in PPROM group average was 254.70 ± 19.44 U / mgprot, in PPROM group average was 241.98 ± 23.55 U / mgprot, the difference between the two groups was not statistically significant (P gt; 0.05). 3 LPO in PROM group and normal group expression levels compared: LPO in normal pregnancy group average is 2.46 ± 0.52 umol / gprot, in PROM group's average is 4.00 ± 1.13 umol / gprot, and normal pregnancy group, the difference was statistically significant (P lt; 0.05). 4 LPO in PPROM group and the expression level of PPROM group compared: LPO in PPROM group average was 4.04 ± 0.97 umol / gprot, in PPROM group average was 3.95 ± 1.29 umol / gprot, the difference between the two groups was not statistically significant (P gt; 0.05). 5 GSH in the PROM group and normal group expression levels compared: GSH in normal pregnancy group average is 123.75 ± 8.98 mg / gprot, in PROM group is the average of 88.52 ± 10.54 mg / gprot, and normal pregnancy group, the difference was statistically significant (P lt; 0.05). 6 GSH in PPROM group and the expression level of PPROM group compared: GSH in PPROM group average was 85.16 ± 10.36 mg / gprot, in PPROM group average was 91.89 ± 9.84 mg / gprot, the difference between the two groups was not statistically significant (P gt; 0.05). 7 MPO in PROM group and normal group expression levels compared: MPO in normal pregnancy group average is 0.11 ± 0.50 U / gprot, in PROM group is the average of 0.20 ± 0.08 U / gprot, and normal pregnancy group, the difference was statistically significant (P lt; 0.05). 8 MPO in PPROM group and expression levels of PPROM group compared: MPO in PPROM group average was 0.21 ± 0.09 U / gprot, in PPROM group average was 0.20 ± 0.08 U / gprot, the difference between the two groups was not statistically significant (P gt; 0.05). 9 Pearson correlation analysis: PROM group and normal pregnancy group SOD and LPO (r = -0.438, P lt; 0.01), MPO (r = -0.393, P lt; 0.01) showed a significant negative correlation between. With GSH (r = 0.677, P lt; 0.01) showed a significant positive correlation. GSH and LPO (r = -0.525, P lt; 0.01), MPO (r = -0.513, P lt; 0.01) showed a significant negative correlation. Conclusions: 1 LPO and MPO expression levels in PROM group than normal pregnancy, premature rupture of membranes showed reactive oxygen species and oxidative stress when the product formation increased. 2 SOD and GSH levels in PROM group was lower than normal pregnancy group, indicating that the antioxidant defense system when PROM weakening. Three groups each index in premature rupture of membranes and preterm PROM group showed no significant difference, indicating the occurrence of oxidative stress has nothing to do with the gestational age. 4 oxides and antioxidant defense system between the balance is broken, oxidative stress generated, and in premature rupture of membranes plays an important role. 5 oxidative stress increases with the oxide formation, decreased antioxidants, antioxidant defense system weakened, leading to oxidative stress generated body function impaired fetal membrane rupture.
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