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Objective: chronic renal failure (Chronic renal failure CRF) cardiac troponin I (Cardiac troponin cTnI) in rat myocardial injury, intact parathyroid hormone (Intact parathyroid hormone, iPTH) and leptin changes explore the role of chronic renal failure in rats with myocardial injury occurred in iPTH with leptin, to provide a theoretical basis for the early diagnosis and treatment of chronic renal failure, myocardial damage. Methods: Platt method to the production of CRF rat model. 48 clean female Wistar rats were randomly divided into three groups: normal group of 12 sham group 12 in the surgery group 24, the surgical group and the sham group underwent the surgery and the sham treatment, Platt law twice after surgery observed 8W. Model operation group of 16 randomly divided into eight 8 model group and treatment group, the sham group 8 rats were used as the control group, which treatment group Rocaltrol the (20ng/100g body weight) dissolved in sesame oil in irrigation stomach, the model group and the control group given equal amount of sesame oil daily gavage for 45d. The three groups of rats after starting treatment once every 15d determination of renal function, the model group and the treatment group rats 5d determination serum cTnI, serum iPTH and serum leptin observed various indicators changes and correlation analysis. Measured by ELISA of cTnI, iPTH and leptin. Statistics using a repeated measures analysis of variance and pearson correlation analysis. Results: 1 CRF rat model to establish: surgery group Platt spell after the 8w last renal function (sCr, BUN) reached the Wistar rat CRF diagnostic criteria: Scr of ≥ 123.8μmol / L and BUN ≥ 8.5mmol / L; kidney light microscope, HE staining showed: slightly thickened glomerular basement membrane, the ball cells were focal segmental hyalinosis, tubular atrophy, and expansion of the coexistence of renal interstitial inflammatory cell infiltration. 2 of the Correlation Analysis: model group: the sCr and leptin, BUN and leptin, sCr of iPTH, BUN of iPTH of iPTH and cTnI, leptin and cTnI showed a straight line is related to the r values ??were: r (Scr, leptin) = 0.948, p lt; 0.01; r (BUN, leptin) = 0.953, p lt; 0.01; r (Scr iPTH) = 0.675, p lt; 0.01; r (BUN iPTH) = 0.426, p lt; 0.01; r (iPTH, cTnI) = 0.691, p lt; 0.01; r (leptin, cTnI) = 0.797, p lt; 0.01. ② treatment group: iPTH of cTnI, leptin and cTnI showed a linear correlation, r values ??were: r (leptin, cTnI) = 0.653, p lt; 0.01; the r (iPTH, cTnI,) = 0.745, p lt ; 0.01. sCr with leptin, BUN and leptin, Scr of iPTH, BUN and iPTH no linear relationship (P gt; 0.01). Conclusion: ① the CRF rats course of early ie myocardial injury, and elevated serum leptin iPTH level. ② calcitriol reduces iPTH and leptin levels, there is a certain improvement in myocardial injury of rats with CRF. The ③ prompt early diagnosis and treatment of secondary hyperparathyroidism and hyperleptinemia delay the progress of CRF.
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