Dissertation > Excellent graduate degree dissertation topics show

The Expression and Clinical Significance of Pim-1 and c-myc in Renal Cell Carcinoma

Author: LuZhiMin
Tutor: QiuJianHong
School: Hebei Medical University
Course: Surgery
Keywords: Renal tumors Clear cell carcinoma pim-1 c-myc Immunohistochemistry
CLC: R737.11
Type: Master's thesis
Year: 2009
Downloads: 73
Quote: 0
Read: Download Dissertation

Abstract


Objective: kidney cancer is common malignant tumor in the Department of Urology, originated in tubular epithelial cells, is the most common renal malignancy, of which approximately 70% to 80% of renal clear cell carcinoma (RCCC), it occurs with The development has been a variety of gene regulation. Therefore, the study of kidney cancer gene to investigate its pathogenesis, diagnostic criteria and treatment of kidney cancer has an extremely important significance. pim-1 is a serine / threonine kinase, it has been confirmed that the amplification or expression of many tumor occurrence and development. The c-myc proto-oncogene c-Myc protein encoded transcription factor of eukaryotic cells, has a dual role of promoting cell proliferation and induce apoptosis. C-myc expression changes can be found in many cancers, such as leukemia, lymphoma, colon cancer, breast cancer. pim-1 and c-myc in apoptosis, proliferation, differentiation and tumorigenesis process has an important role, while studies have shown that both T, B lymphoma and prostate cancer can produce synergy to promote tumorigenesis and development . However, the two proteins expression in RCC and related research at home and abroad has not been reported. This study aimed to detection pim-1 and c-myc expression in renal cell carcinoma during kidney cancer classification and points to explore renal cell carcinoma pim-1 and c-myc expression and cancer tissue infiltration metastasis and prognosis of the relationship, and pim-1 and the correlation between the c-myc, to investigate kidney cancer clinical biology behavior provides new clues and ideas, and to lay the theoretical development of new effective drugs and methods of treatment of kidney cancer foundation. Materials and methods: 1 material: 2004-2008 Bethune International Peace Hospital primary resected RCCC paraffin specimens of 80 cases, including 56 males and 24 females. Age 18-82 years, mean 55.9 years. According to Fuhrman renal cell carcinoma fractionation system is divided into 12 cases in G1, G2 grade 21 cases, 38 cases of grade, G3, G4 grade nine cases. Divided into 11 cases of the Phase I, II, and 24 cases of 36 cases, III, IV of nine cases of kidney cancer at the 2002 American Joint Committee on Cancer (AJCC) clinical staging criteria. Surgery with lymph node metastasis in 38 cases, 42 cases without lymph node metastasis. In another 20 cases of cancer and adjacent tissues and 8 normal renal tissue (cut from other diseases of the kidney) as a control. 2 Methods: Immunohistochemical PV6002 law retrospective study of selected specimens pim-1, c-myc expression, an anti-mouse anti-human pim-1 and c-myc monoclonal antibody, DAB color hematoxylin. Negative control antibody was replaced with PBS. Random selection of the four fields, each field of view observed for 100 cells, according to the staining intensity and range, respectively, divided into A and B, the final score for the AxB. Score ≤ 1 is negative, gt; 1 is positive. 3 Statistical analysis: application SPSS13.O statistics software for data processing, the positive rate between the two groups was used to compare the X2 test (P lt; 0.05) between pim-1 and c-myc expression correlation using the Spearman rank correlation analysis. Results: 1 pim-1 staining in the cytoplasm, nucleus and cell membrane rarely expressed. 80 cases RCCC, pim-1 positive expression in 69 cases (86.7%); 8 patients with normal renal tissue and 20 cases of paraneoplastic positive expression were 2 cases (25%) and 7 patients (35%) of RCCC group The other positive expression rate difference was significant (P lt; 0.01), and cancer paraneoplastic positive expression rate of the normal kidney tissue was no significant difference in sex (P gt; 0.05). 2 c-myc staining mostly localized in the cytoplasm, a few are located in the nucleus. The RCCC in 80 cases, c-myc-positive expression in 68 cases (85%); 20 paracancerous positive expression in 6 patients (30%), 8 patients with normal renal tissue positive expression in 2 cases (25%). The RCCC group with other positive expression rate difference was significant (P lt; 0.01). Cancer paraneoplastic normal kidney tissue positive expression rate difference was not statistically significant (P gt; 0.05). 3 pim-1 and c-myc expression with patient age, gender, no significant correlation with the clinical stage and pathological grade and pim-1 and c-myc expression in renal cell consistent: they I-11 (P lt; 0.05), the expression level was significantly higher than in the G3-G4-class level G1-G2 (P lt; 0.05) in the metastatic lymph nodes was significantly higher than in the III-IV period RCCC expression was significantly higher than that of the organization without lymph node metastasis (P lt; 0.05). Statistically analyzed pim-1 and c-myc expression in renal cell carcinoma were positively correlated (r = 0.727, P lt; 0.01). Conclusion: pim-1 renal cell carcinoma, the expression of c-myc and paraneoplastic tissue, corresponding indicators in normal renal tissue expression of the existence of significant differences (p lt; 0.05) shows that pim-1, c-myc may the occurrence of kidney cancer are closely related. 2 pim-1 and c-myc expression in kidney cancer pathological grade, clinical stage and lymph node metastasis between the existence of significant differences (p lt; 0.05), suggesting that the pim-1, c-myc may in kidney the occurrence of cancer, plays an important role in the development of its protein detection as the determination of an objective indicators of renal cell carcinoma and invasion and metastasis. 3 kidney cancer pim-1 and c-myc expression was positively correlated synergies may exist, both in the process of kidney cancer. 4 pim-1 with abnormal expression of c-myc in renal cell kidney cancer diagnosis and treatment and determine prognosis provide a theoretical basis.

Related Dissertations

  1. Protein phosphatase 2A Cα subunit knockout cardiac energy metabolism due to remodeling,Q78
  2. Some Fluorescence Quenching Spectral Analysis Interacted with Bio-related Molecules,X832
  3. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  4. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  5. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  6. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  7. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  8. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  9. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  10. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  11. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  12. Construction and Identification of SHH-N Gene Adeno-associated Virus Vector and Its Effection on Genes Related to Proliferation in Neural Stem Cells,R329
  13. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  14. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  15. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  16. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  17. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  18. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  19. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  20. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1
  21. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8

CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
© 2012 www.DissertationTopic.Net  Mobile