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The Effects of Selective Cerebral Ultra-profound Hypothermia on Cytoskeletal Protein in Monkeys after Occlusion of Cerebral Blood Flow under Different Time Limits

Author: LiRan
Tutor: XuWei
School: Kunming Medical College
Course: Surgery
Keywords: Cytoskeletal proteins Ultra - deep hypothermia Monkey brains Clinopodii
CLC: R651
Type: Master's thesis
Year: 2009
Downloads: 46
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Abstract

To observe room temperature ischemic monkey brain 0 minutes, 10 minutes, 15 minutes, 20 minutes after selective cerebral ultra-deep cryogenic recovery of brain cytoskeletal proteins β-tubulin, MAP-2, vimentin and GFAP expression changes to Learn selective deep hypothermia recovery after cerebral ischemia skeleton of monkey neurons and glial cells, and to explore the monkey brain the room temperature ischemic recovery limit of time. 【Methods】 15 healthy adult rhesus monkeys, male, were randomly divided into four groups, group 0 minutes (bilateral carotid artery occlusion immediately ultra deep hypothermic perfusion group, referred to 0 minutes group, n = 4), 10-minute group ( bilateral carotid artery at room temperature blocked 10 minutes of deep hypothermic perfusion group, referred to as the 10-minute group, n = 4), 15 minute group (bilateral carotid artery at room temperature to block the 15 minutes of deep hypothermic perfusion group, referred to as the 15-minute group, n = 4), 20 minutes (bilateral carotid room temperature blocking 20 minutes of deep hypothermic perfusion group, referred to as the 20 minutes group, n = 3). Right internal carotid artery intubation connected to the distal end of the cold perfusion system, the proximal end of the distal end of the right internal jugular vein and bilateral femoral vein catheter connection ultrafiltration rewarming, cerebral cardiopulmonary bypass. Clamping bilateral neck arteriovenous arteriovenous room temperature to 4 ° C Ringer's solution is injected into the right internal carotid artery after ischemia in the side of the neck, to maintain the brain temperature ≤ 16 ℃ 60 minutes. Cranial MRI examination before and after surgery, postoperative neurologic deficit score. Craniotomy brain immediately after the death of the the experimental monkey perfusion or recovery, long-term survival of monkeys reared sacrificed at 4 weeks after craniotomy brain. The vertical chemical method of immunohistochemical detection of nerve cells in the β-tubulin, MAP-2 and glial cells Vimentin and GFAP protein expression levels. [Results] 0 minutes group (n = 4), 10 minute group (n = 4) the safety recovery and long-term survival, intraoperative and postoperative hemodynamic stability, postoperative brain MRI no abnormal postoperative neurologic function rated no exception. Long-term survival of two 15-minute group (n = 4), a severe disability and death one. 20 minutes after perfusion group failed safe recovery of all deaths. Immunohistochemical results showed that 20 minutes group and 15 minutes and 0 minutes group and 20 minutes groups and group 15 minutes compared with 10 minute group, neurons tubulin, β-tubulin, the MAP-2 expression decreased P <0.01 Vimentin of glial cells and GFAP expression was significantly higher P <0.01 10 minutes and 0 minutes group and 20 minutes group compared with the 15-minute group difference was not statistically significant. [J perish knot] (1) primates rhesus unilateral internal carotid artery cold perfusion conditions quickly achieve selective cerebral ultra-deep hypothermia (14.3-16 ° C). (2) blocking simian bilateral internal carotid artery blood flow at room temperature for 0 minutes, 60 minutes after the line 10 minutes after selective cerebral ultra deep hypothermic perfusion safe recovery and long-term survival of the hemodynamic brain tissue and nerve function, no significantly affect, the ultra-deep hypothermia the obvious brain protection role. (3) at room temperature 15 minutes of ischemia underwent selective deep hypothermia recovery is possible, but the high rate of mortality and disability monkeys. Hypothermic perfusion after cerebral ischemia 20 minutes at room temperature, and the experimental monkeys can not be recovery. Cerebral ischemic neuron damage appears irreversible brain damage, neuron micro tubulin β-tublin Map-2-disintegrating increased, hyperplasia and hypertrophy of glial cells, glial cell marker protein GFAP and Vimentin expression was significantly increased . (4) the brain room temperature low temperature recovery after ischemia prime time may be for 10 minutes. Low temperature recovery after 15 min ischemia, which is likely to save lives, but neurological deficits and higher rates of mortality and disability, there is a big recovery in risk.

The Effects of Selective Cerebral Ultra-profound Hypothermia on Cytoskeletal Protein in Monkeys after Occlusion of Cerebral Blood Flow under Different Time Limits

Abstract

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