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Objective: To investigate autophagy gene Beclin-1 and microtubule-associated protein LC3 Expression and significance of cardiomyopathy in rats with doxorubicin (Adriamycin, ADR), initially confirmed autophagy (Autophagy) involved in adriamycin myocardial the incidence of the disease, and to explore its mechanism, and provide a theoretical basis for the prevention and treatment of clinical doxorubicin cardiomyopathy. Methods: Male Sprague (Sprague-Dawley) SD rats 45, weighing between 180 to 200 grams, were randomly divided into three groups, adriamycin (ADR) group (N = 15), A adriamycin (ADR) - A -adenine (3-Methyladenine ,3-MA) ??group (N = 15), the control group (N = 15). Adriamycin (ADR) Group: experiment the day after the intraperitoneal injection of doxorubicin 4mg/kg, once a week, a total of six weeks, the cumulative dose 24mg/kg; adriamycin (ADR) 3 - methyl adenine ( 3-MA) ??group: the next day of the experiment from the intraperitoneal injection of the same dose of doxorubicin, intraperitoneally injected half an hour before the injection of autophagy inhibitor 3 - methyladenine (3-MA) ??500nmol (5ul); control group: intraperitoneal injection of saline. Sixth weekend of the experimental rats were weighed and echocardiography measurements of left ventricular systolic function (ejection fraction, EF and fractional shortening FS), malondialdehyde (malondialdehyde, MDA) in plasma and whole blood, heart blood spectrophotometric detection prototype glutathione (reduced glutathione, GSH) content, take heart and weighed calculated heart weight index (heart weight index, HWI), take a left ventricular myocardial specimens, HE (with hematoxylin and eosin) staining myocardial pathological changes, electron microscopy since autophagy gene Beclin-1 content, morphology and the number of phages immunoblotting (west-blotting) detect myocardial tissue immunohistochemistry SP method cardiomyocytes microtubule-associated protein (Microtubule-associate Protein 1Light Chain 3, LC3 ) content. All data are used SPSS11.5 statistical software for statistical analysis. Results: 1, ADR group left ventricular systolic function compared with the control group decreased (P <0.05), a statistically significant; in ADR group left ventricular mass index (HWI) higher than the control group, P <0.05, statistically significant; MDA level in ADR group than the control group increased, P <0.05, statistically significant, whole blood GSH content in ADR group than the control group to reduce, P <0.05, statistically significant; 3, Western blotting: ADR Group Beclin-1 levels in the myocardial tissue increased compared with the control group and the ADR 3-MA group, P <0.05, statistically significant; 4, immunohistochemical staining: ADR group myocardial tissue in LC3 content compared with the control group and ADR 3-MA group increased (P <0.05), with statistically significant; 5, linear correlation analysis: autophagy gene Beclin-1 levels of microtubule-associated protein LC3 content was significantly positively related. Conclusion: 1, doxorubicin can cause myocardial tissue of rats autophagy gene Beclin-1 level increases, thereby inducing myocardial autophagic programmed cell death, leading to the occurrence of myocardial lesions. 2, doxorubicin cardiomyopathy rat cardiomyocytes microtubule associated protein LC3 was significantly increased, and the autophagy gene Beclin-1 level was significantly positively related. 3, autophagic programmed cell death (Autophagy) as another outside of necrosis and apoptosis programmed cell death (programmed cell death, PCD) involved in the pathogenesis of doxorubicin cardiomyopathy can be autophagy inhibitor 3-MA suppressed.
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