Dissertation > Excellent graduate degree dissertation topics show

The Study on Pathogenicity of CHK Mutants in Candida Albicans

Author: ChenMeiJing
Tutor: ChengBo；ZhangSheng
School: Fujian Medical
Course: Pathology and Pathophysiology
Keywords: Candida albicans chk kinases Candidiasis Animal models Histological techniques Ultrastructure
CLC: R379
Type: Master's thesis
Year: 2009
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


Objective To compare Candida albicans CHK mutant CHK26 in (CHK Ser / Thr MAPK and GAF ??domains deficient strain), CHK21 (CHK allelic deletion strain) and standard strain CAF-2 pathogenicity in mice to understand CHK Its domain of the role in the pathogenicity of Candida albicans. Method 1. Establish a mouse model of systemic Candida albicans infection: 18 clean healthy Kunming mice were randomly divided into three groups A, B, C, n = 6, respectively, by tail vein injection of C. albicans CAF-2 , CHK26, and CHK21 overhang the broth 0.5ml (1 × 10 6cfu). Remove the mouse kidney fungal culture do histopathological examination and renal tissue. Mortality in mice, and the survival of protected time experiment: ibid constructed mouse model 63, the morbidity and mortality of the mice in each group (n = 21), twice daily observation. Renal histopathology and electron microscopy detection: to build mouse model 12, n = 4. 3 day and 5 days a mice model kidney, histopathology, PAS staining, another kidney produced electron microscopy specimens were observed under transmission electron microscope. 4. Spleen and kidney tissue colony forming units (cfu): mouse model 45, 15 in each group. 1 day, 3 days and 5 days out made of five mice of both kidneys and spleen homogenates After incubation, count the number of colonies. Compare each group of mice spleen and kidney tissue colony forming unit count differences. 5 standard strains of Candida albicans, CHK the mutant CHK26 and CHK21 preparation electron microscopy and scanning electron microscopy specimens, electron microscopy ultrastructure. Results 1 successfully established a mouse model of systemic Candida albicans infection: three groups of mice kidney histopathology PAS staining visible obvious hyphae and spores, kidney tissue culture to see the growth of yeast-like colonies. Experimental results on mortality and survival protection: standard strain, CHK26 the CHK21 infected mice observed during mortality were 100%, 23.8% and 0 the; CHK21, CHK26 infected mice survival time was significantly longer than the standard strains of mice, statistically significant. 3. The kidney histopathological and electron microscopy test results: kidney tissue pathology detection: the standard the strain, CHK26 and CHK21 infected mice kidney tissue biopsy PAS staining visible fuchsia hyphae and spores. The transmission electron microscope to see: three Candida albicans infection in renal tissue were seen in the epithelial cells of Candida albicans spores. Standard strains of mice infected kidney tissue ultrastructure change significantly, showing tubular epithelial cell surface microvilli shedding even focal necrosis; epithelial cells mitochondrial swelling; renal interstitial neutrophil infiltration ultrastructural changes. CHK26 mice infected kidney tissue lesions lighter, individual tubular epithelial cell edema; lysosomes; renal interstitial infiltration of neutrophils. CHK21 infected mice kidney tissue structure generally normal; only see the individual epithelial cell swelling; small amount of neutrophil infiltration. 4. Spleen and kidney tissue colony forming units (cfu) Results: Candida albicans infection in mouse spleen and kidney tissue colony count results as shown in Table 3: CHK21 clear the slowest in the spleen and kidney tissue, CAF-2 Clear fastest, CHK26 The between standard strains and CHK21. Candida albicans transmission electron microscopy see CHK26 cell wall thickness varying electron-dense outer layer and a transparent inner layer is significantly less than the CAF-2, or even part of the cell wall disappear; basic integrity of the cell membrane. Complete CHK21 cell wall of varying thickness, the thickness in between. The scanning electron microscope, see three Candida albicans round or oval shape, smooth surface; the standard strains CHK26 strains seen in the major axis ends of the cells budding; while the surface of the CHK21 strain cells bud scar random distribution. Conclusion 1. CHK Candida albicans virulence play an important role, CHK21 performance is non-toxic, CHK26 manifested as reduced virulence in a mouse model. Candida albicans infection of a mouse model of renal tissue lesions mainly in the renal tubules. Mice infected with Candida albicans, renal tissue in renal tubular epithelial cells engulf Candida mitochondrial swelling, renal interstitial neutrophil cell infiltration ultrastructural changes mainly. 3. CHK affect Candida albicans cell wall construction, may also be virulence reduce the main reason. 4. Ser / Thr MAPK and GAF ??domains in Candida albicans virulence and cell wall building plays an important role. Changes in the structure of the cell wall, it may affect the budding of Candida.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. Effect of Fasting on Rigor Mortis and Meat Quality of Chicken Breast Muscle,TS251.1
  3. The Effects of Aerobic Exercise Training on Oxidative Stress and Ultrastructure of Pancreatic Organization in Diabetic Rats,R587.1
  4. Changes of Cell Ultrastructure and Physiological Indexes and Physical Properties of Tobacco Leaves during Bulk Flue-Curing,S572
  5. Different rice varieties to brown planthopper resistance mechanism Preliminary Study,S511
  6. Oral epithelial cells were infected with Candida albicans after HBD-2 mRNA and protein expression in vitro,R780.2
  7. Genotyping and Antifungal Susceptibility Testing of Candida Albicans from Patients with Atrophic or Erosive Oral Lichen Planus,R781.5
  8. Diffusion Tensor Imaging Using 3.0T MR Scanner in Rat C6 Glioma Model and the Correlative Study of FA Value in Infiltrated Peritumoral Edema Tissue and AQP1,R739.4
  9. The Impact of Estrogen on the Expression of HWP1 Gene of Candida Albicans,R711.3
  10. Research the Characteristic of the Traditional Meat (Roasted Chicken) During Processing,TS251.67
  11. Morphological Study of Yak Placenta During Gestation,S823.85
  12. Mutagenic Effect of Satellite Carrying for Trifolium Repens,S541.2
  13. Combined Use Direct Endotoxin Absorption and High Volume Hemofiltration on the Influence of Endothelial Cells,R459.7
  14. Diffusion-weighted Imaging and Apoptosis Research in Rabbit Models of Hepatic VX2 Carcinoma After Radiotherapy,R735.7
  15. A Study of the Histologic Lesion Variability by the 125-iodine Radion Implanted of Hepatoma and Normo-liver,R735.7
  16. Intrathecal chloroprocaine neurotoxicity experimental study,R614
  17. Genotyping of Oral Candida Albicans Isolated from HIV-infected Patients,R512.91
  18. The Resistance of Candida Albicans to Common Antifungal Drugs and Its Expression of CDR1 and CDR2 Gene,R440
  19. Study of Real-time Quantitative PCR on the Early Diagnosis of Deep-seated Candida Infections,R450
  20. Different Frequencies of Electroacupuncture Pretreatment on Rabbit Model of Acute Myocardial Ischemia Comparison of Protective Effect,R245
  21. Self albicans treating erythema ( atrophy ) type of oral Candida albicans infection clinical observation,R276.8

CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic fungi ( molds) and actinomycetes
© 2012 www.DissertationTopic.Net  Mobile