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Studies on the Chemical Constituents and Antitumor Activity of Forsythia Suspense (Thunb.) Vahl.

Author: MaoWei
Tutor: WangXiaoMing
School: Hubei College of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Forsythia Chemical composition Structural identification MTT assay The anti-tumor activity
CLC: R284
Type: Master's thesis
Year: 2009
Downloads: 336
Quote: 4
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Abstract


Forsythia Oleaceae plant forsythia ( Forsythia suspense ( Thunb.) Vah1 (Oleaceae) ) dried ripe fruit , with Qingrejiedu Sanjie swelling of the effect , the Indications warm , erysipelas , spotted , carbuncle ulcers swollen poison M. scrofulaceum , urine poured off embolism . The forsythia ethanol extract is one of the commonly used anticancer Prescription drugs , clinical , often made ??of forsythia and other Chinese herbal formula, and the treatment of head and neck cancer , esophageal cancer , stomach cancer , breast cancer , cervical cancer , leukemia and more kinds of tumors , have been reported in the literature the forsythia ethanol extract has broad-spectrum anti- tumor activity of a variety of tumor cells significantly inhibited . In order to explore its anti-tumor active substance basis , this experiment on the basis of preliminary studies , the use of the system solvent and chromatographic separation of the strongest activity of the ethyl acetate portion was separated , separated from the active portion 12 of the monomer compound , structure identification of these types of compounds with chemical and spectroscopic methods were betulinic acid ( Betulinic acid ) , oleanolic acid (Oleallolic acid), ursolic acid (orsolic acid), 2α ,23-OH ursolic acid the (2α ,23-OH, ursolic acid), phillyrin ( phillyrin ) , forsythia lignans (phillygenin), table pinoresinol (epipinoresinol), different the larch the lignans ( isolarlcires inol ) daucosterol ( daucosterol ) , β - amyrin acetate ( β - Amyrinacetate ) , β - sitosterol ( beta -sitosterol ) , Gok mesothelin ( quercetin ) . Isolated and identified three lignin epoxy monomer compounds forsythia V ( phillyrin ) the forsythia the the visfatin VI ( phillygenin ) , Table pinoresinol VII ( epipinoresinol ) of these three kinds of compounds were observed by MTT assay inhibition of growth of human gastric cancer cell line SGC7901 the certain extent forsythia lignans and table the pinoresinol of growth of human gastric cancer cell line SGG7901 , , while inhibition phillyrin obvious . The above findings lay the foundation for further study in Forsythia active substances epoxy lignin substances .

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