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Nettle (Urtica fissa E. Pritz.), Also known as lobed leaves nettle, nettle nettle species, is China's commonly used in folk medicine and folk medicine. Have chills and scared, Warming digestion, for the treatment of rheumatism, sprains, pain and itchy skin, postpartum convulsions, convulsions in children, urticaria and other diseases. Urtica plant varieties in China, widely distributed, abundant resources. The biological characteristics of stems and leaves covered with sting hair, skin contact immediately aroused strong stimulus, itchy swelling and pain, blistering serious that it fester. The nettle has always been at arm's length, perhaps for this reason that, as of the beginning of this century, domestic nettle species little studied, these resources are still in a state to fend for themselves, foreign, especially German scholar of nettle (also known as dioecious nettle) found that the treatment of benign prostatic hyperplasia, attracted wide attention from scholars at home and abroad. Nettle in our country is rich in resources, 16. The present study had pre-six kinds of domestic nettle plant of the genus different extracts of biological activity screening of the anti-prostate hyperplasia, the results show that 20% and 95% ethanol extract of nettle root can significantly inhibit prostate hyperplasia in rats diseased tissue. 5 alpha-reductase is a key enzyme in the formation process of benign prostatic hyperplasia, can promote the conversion of testosterone to dihydrotestosterone, DHT is an important factor leading to benign prostatic hyperplasia, therefore, inhibition of 5a-reductase activity of the treatment benign prostatic hyperplasia key. In this study, the enzyme as a target established by enzyme-linked immunosorbent assay 5 alpha-reductase inhibitors in vitro models of different polar fractions collected from the Jinchuan County, Sichuan Province, nettle root extract, 5 alpha-reductase inhibitory activity, the results showed that the 95% ethanol extract of nettle root of n-butanol extract significantly inhibit activity. This article focuses on the nettle 95% ethanol extract of the chemical composition of the polar parts of the system. By silica gel column chromatography, thin layer chromatography, macroporous resin column chromatography, reverse phase silica gel column chromatography, HPLC separation method 18 compounds were obtained from which the separation, and the use of modern spectroscopic methods (NMR, MS, etc.), the standard product control, reference data control and determination of physical and chemical constants, identified 14 structures of the compounds. : Cis-3-O-beta-glucose-methyl-4 - [bis (3 - methoxy - 4 - hydroxyphenyl) methyl] butyrolactone (1), anti-3-O-beta- glucose-methyl-4 - [bis (3 - methoxy - 4 - hydroxyphenyl) methyl]-butyrolactone (2), anti-3-O-beta-glucose-methyl-4 - [bis (3, 4 - dimethoxyphenyl) methyl]-butyrolactone (3), anti-3-O-beta-glucose-methyl-4 - [(3 - methoxy - 4 - hydroxy-phenyl-3 ,4 - dimethoxyphenyl) methyl]-butyrolactone (4), alpha-fragrant resin alcohols (5), the beta-balsams alcohol (6), oleanolic acid (7), bis (5 - formyl furfuryl) ether (8), 5 - the hydroxymethylfurfural (9), 4 - hydroxy-benzoic acid (10), scopoletin (11), scopolamine glycosides (12), daucosterol (13), beta- sitosterol (14). Wherein the compound 1 to 4 are novel compounds; compounds 1 to 11, were first isolated from the nettle plants.
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