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A mutant Arabidopsis leaf curl Genetic Analysis and Gene Mapping

Author: YuanYuJu
Tutor: ZuoShengï¼›LiDongPing
School: Hunan Normal University
Course: Botany
Keywords: Arabidopsis lcd mutant Leaf curl IAA Map-based cloning SSLP
CLC: Q943
Type: Master's thesis
Year: 2009
Downloads: 149
Quote: 0
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Abstract


Ye are the basic plant vegetative organs . Leaf development by co-regulated genes and environmental factors . Leaf polarity establishment directly determines the leaf level malleable development, polarity change will destroy most plant leaves flat malleable , affecting plant photosynthesis , transpiration and resistance and other physiological functions. Learn leaf or leaf curl occurs polar genetic law , regulation mechanism for the study of leaf development has a very important theoretical significance. In this thesis, a method of forward genetics abnormal leaf development caused by mutations in a gene function , map-based cloning methods lcd from Arabidopsis mutant clones corresponding gene . The main results and conclusions are as follows: 1 ) mutant morphology : lcd mutant rosette leaves in horizontal and vertical are different degrees of curl , and the more the greater the degree of curl in the early to the late curl leaf development was significantly smaller. At the same time , leaf angle becomes large. There internode developmental stages of flowers appear and bending angle of pods with students has also changed. 2 ) study the mutant hormone responses respectively mutant and wild -type in the role of different concentrations of growth hormone , found in high concentrations of mutant IAA IAA under treatment inhibited than the wild -type sensitive . 3 ) for the genetic analysis of mutants , the mutant with wild -type hybridization , selfing . F1 showed wild-type traits , F2 segregating appear , F2 populations of wild-type and mutant trait trait segregation ratio of 3:1 plants , indicating that the mutation is recessive mutation in a single gene . 4 ) the use of map-based cloning technology, has mutated gene was located in an Arabidopsis chromosome III , and molecular markers tightly linked GAPAB .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics
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