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Objective To study the phenytoin (Sodium phenytoin, PHT) drugs in human periodontal ligament cells by expression of tensile strain role to further explore the subject in periodontal ligament cells proliferation, the phenytoin its promoting proliferation of drugs, so as to clinical orthodontic force due to an excessive damage caused by periodontal solution provides new ideas for further research PHT for periodontal wound healing and tissue regeneration provides experimental data to support the role. Methods The clinical result of the normal removal of orthodontic treatment premolars, the use of tissue adherent cells were cultured periodontal ligament. Preparation 20,50,100,500,2500 μg / ml of different concentrations of PHT culture fluid. Histocompatibility use silicone membrane of cultured human periodontal ligament cells. Use of biological tissue testing device for multi-modal silicone membrane stretch. Application tetrazolium colorimetric test method (MTT method) were used to detect different concentrations of PHT (20,50,100,500,2500 ug / ml), 10%, 15%, 20% tensile strain and tensile strain effect effect after 4h adding a certain concentration of PHT on periodontal ligament cell proliferation. Using SPSS software for single-factor analysis of variance. Result 1: tissue adherence assay results: Bosi protein positive, anti-keratin negative. 2: PHT in 20,50,100 ug / ml concentration, no significant changes in cell morphology; when the concentration reaches 500,2500 ug / ml, the cells showed a toxic effect, the cell structure is destroyed, showing crystalline substance. 500ug/ml when a small number of adherent cells; 2500ug/ml, the non-adherent cells. MTT added after 4h, DMSO terminate hole with varying concentrations of purple needle crystals formed. MTT assay also confirmed: 20ug/ml concentrations, PHT on periodontal ligament cells significantly promoted proliferation. 3:10%, 15%, 20% tensile strain 4h, microscope, showing periodontal ligament cells adherent good cell growth compared with the control group had no significant change of direction. MTT assay showed: 10% tensile strain can significantly promote hPDLC proliferation, with the control group there was significant difference (p lt; 0.05). 15%, 20% tensile strain under hPDLC proliferation was inhibited, with the control group there was significant difference (p lt; 0.05). 15%, 20% tensile strain between the two no significant difference (p gt; 0.05). 4:20 ug / ml of PHT can significantly reverse the excessive tensile strain as a result of hPDLC proliferation: the role of the 15% tensile strain of hPDLC join 20ug/mlPHT 4h incubated 20h, by MTT assay, cell proliferation significantly, with the blank group significant difference (p lt; 0.05), with 15% tensile strain 4h group had significant difference (p lt; 0.01). Conclusions 1. Different concentrations of PHT on periodontal ligament cell proliferation was significantly different. (2) adequate tensile strain can promote periodontal ligament cells, excessive tensile strain on the periodontal ligament cells proliferation. 3. Appropriate concentration of PHT due to being too large tensile strain arising from proliferation of periodontal ligament cells to reverse the pro-proliferation.
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