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Studies on the Bacterial Infection and Its Pathogen of Crab (Eriocheir Sinensis L.)

Author: FangHai
Tutor: ZhaoXianJun；ChenCuiZhen
School: Northwest University of Science and Technology
Course: Agricultural Extension
Keywords: Chinese mitten crab Pathogenic bacteria Test
CLC: S945
Type: Master's thesis
Year: 2008
Downloads: 66
Quote: 1
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Abstract


Hebei Qinhuangdao, Tangshan aquaculture farms, 2 (NO.1, NO.2) Annual Chinese mitten crab (Eriocheirsinensis H.Milne-Edwards) disease, the test, the results showed that both bacterial septicemia infections. NO.1 anguillarum Vibrio (Vibrio Pacini 1854) (V.anguillarum), Aeromonas (AeromonasKluyver and van Niel 1936) Vickers of Aeromonas (A.veronii) and citric the Bacillus (GitrobacterWerkman and Gillen 1932) Citrobacter freundii (C.freundii), mixed infection; NO.2 anguillarum infection alone. Separation do pure culture 24 bacterial morphological characteristics, physical and chemical characteristics of the system's apparent classification to learn indications identification and on behalf of the G Cmol% Determination of strain in DNA; while selecting representative strains of the 16S rRNA gene molecular identification and determination of the 16S rRNA gene sequence analysis the corresponding sequence homology of the associated bacteria, a phylogenetic tree was constructed. 6 (HQ0105016B-tested anguillarum 12 (NO.1 Case 6: HQ0105016A-1, to HQ0105016A-6, 6 NO.2 cases: HF010522-1, to HF010522-6), Vickers Aeromonas 1 to HQ0105016B-6) and Citrobacter freundii 6 (HQ0105016C-1 to HQ0105016C-6); selected representatives the strains HQ0105016A-1 and HF010522-1 anguillarum HQ0105016B-1 (Vickers Aeromonas bacteria measured) and the 16S rRNA gene sequence HQ0105016C-1 (Citrobacter freundii) results of the amplification of the 16S rRNA gene sequence length: HQ0105016A-1 strain 1423bp (GenBank accession number DQ068758), HF010522-1 Zhu 1420bp (GenBank accession number DQ068759), HQ0105016B-1 strain 1413bp (GenBank accession number DQ010114) of HQ0105016C-1 strain 1420bp (GenBank accession number DQ029351). Clinical characteristics of the cases from the Chinese mitten crab, pathological changes in inspection; slide agglutination, indirect fluorescent antibody technique to represent the strain corresponding antisera prepared by immunizing rabbits serological test, test the clear serum homology detection of pathogens; choose isolates representative strains to do health crab artificial infection test, initially clear corresponding etiological significance of the isolates. Inspection techniques to establish the characteristics of the disease, pathogenic bacteria were isolated and identified immunological test; made against the fungus drug susceptibility testing of isolates, isolates resistant spectrum initially clear. The research results on the crab corresponding bacterial septicemia infection test the etiology of the disease and its prevention and control has certain reference value.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Fisheries Protection > Pest and Disease Control of the crustaceans
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