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This study mainly Festuca wild species as a material in China's Yunnan , Guizhou and Sichuan three provinces 10 counties around 100 points grass plants sheath detection of endophytic fungi were field tested five genus 14 kinds of 1091 plants , which detected 628 fescue has 421 containing endophyte infection rate of 67%. More than a thousand seeds were collected , of which there are eight kinds of seeds contain hyphae. In this study, using different separation methods successfully isolated two fescue Festuca ovina and wild species Festucastapfii endophyte two kinds of 90 strains , and in the MEA, MM medium and induced under different treatments conidia , initially identified as N.typhinum and N.starrii, in Festuca stapfii isolated N.starrii also belong to the first in the country . On isolated two strains N.typhinum, N.starrii bacteriostatic experiment , N.typhinum and N.starrii for leaf spot, Pythium pathogens antagonistic inhibition rates were 36.8% , 59.1% , 34.2% , 43.2% . N.typhinum the difficulty with N.starrii volatile metabolites on leaf spot, Pythium pathogens antagonistic inhibition rates were 16.2% , 18.4 %, 21.6 %, 26.7% . But N.typhinum, N.starrii of volatile metabolites Quedui leaf spot, Pythium no significant inhibitory effect . Select type tall fescue turf of seven varieties Millennium, Tripe A, Arid Ⅲ, Eldorado, Crossfire Ⅱ, Little Hero, Sun Boy and pasture -type tall fescue Fawn, broken seeds soaked with bacteria France , sterile seedling injection, adult mycelium stuffing method endophytic fungi inoculation studies . Among adult mycelium stuffed Law does not get vaccinated success. Broken seeds soaked successful tieback bacteria France was 2.1 % sterile seedling success tieback injection rate of 2.5 %, and eight species have gained a new commodity symbiont , the infection rate was 2 %, 1.20 % 3.50% , 5.20% , 1.50% , 0.50% , 0.50% , 5.70% .
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