|
Lily (Lilium spp.) Are monocots subclass Liliaceae (Liliaceae) Lilium (Lilium) perennial bulbous plants, suitable for use as cut flowers, potted and landscape scenery, with high ornamental and commercial value, loved by the people . Carry polyploid breeding is to improve the quality and resistant ornamental lily a very important way. China has abundant resources of wild lily, but not yet fully exploited, this test on 10 kinds of Chinese origin imported goods varieties of wild lilies and tissue culture system for a comparative study and use of colchicine and oryzalin on which the ornamental value And no reports successfully induced polyploid wild species Qingdao lily (Lilium tsingtauense), Tong Jiang lily (Lilium sargentiae), Lilium (Lilium pumilum) were polyploid induction, hope I can get a larger flower diameter, plant more robust lily new germplasm, new materials, abundant resources, the gene pool of lilies, lily of the times for the breeding and our own intellectual property rights lay the foundation for the cultivation of lily varieties, and is now widely used on the market, but resistance poor lily cultivar 'Siberia' ('Siberia') for polyploid induction, you can expect to get high ornamental value, strong resistance to new germplasm. The main results are as follows: (1) through research, the establishment of 10 kinds of lily in vitro regeneration system. 'Siberia' ('Siberia'), 'Marshal' ('Acapulco'), 'Yellow Storm' ('Yelloween') optimal induction of differentiation and proliferation medium for MS 6-BA2.0mg / L NAA0.5mg / L, induction rate, the multiplication coefficient reached 92.16%, 90.09%, 85.56%, 3.87,3.54,3.70. Spotted lily (L.concolor var.pulchellum), Lilium and optimal induction of differentiation and proliferation medium was MS 6-BA1.0mg / L NAA0.1mg / L, induction and multiplication coefficients were 68.26%, 60.21 %, 4.34,4.56. 'Whitehaven' ('White-heaven') the optimal induction of differentiation medium was MS 6-BA1mg / L NAA0.1mg / L, induction rate was 80.43%, Lanzhou lily (L.davidii var.unicelor) optimal induction of differentiation medium was MS 6-BA1mg / L NAA0.5mg / L, induction rate was 95.23%, the volume of Dan (L.lancifolium), Tong Jiang lily bulbils optimal induction of differentiation medium was MS 6-BA1mg / L NAA0.1mg / L, induction rate was 87.44%, 92.00%. Qingdao lily, Lilium optimal proliferation medium was MS 6-BA1mg / L NAA0.1mg / L, multiplication coefficients were 1.49,5.97, lancifolium optimal proliferation medium was MS 6-BA1mg / L NAA0.5mg / L multiplication coefficient of 2.48, Tongjiang lilies and 'Whitehaven' optimal proliferation medium was MS 6-BA0.5mg / L NAA0.1mg / L, multiplication coefficient 3.02,3.00. 1/2MS NAA0.1mg / L as more appropriate lily rooting medium. (2) there are spots on the lily, Lilium, lily Tongjiang, 'Whitehaven' conducted callus induction test. Get spotted lily, Lilium scales best medium for callus occurred MS 6-BA2mg / L NAA0.5mg / L, the rate was 57.00 percent more, 60.67% Tongjiang lilies and 'Whitehaven' scales callus occurs best medium for MS 6-BA1mg / L NAA0.5mg / L, the callus was 60.93%, 67.6%. Through three factors and three levels orthogonal test MS KT1.5mg / L 2,4-D1mg / L NAA1.5mg / L of Lilium, lily Tongjiang optimum blade callus, in which the concentration of leaf KT Callus greatest impact. Spotted lily, Lilium optimum callus proliferation and differentiation medium was MS 6-BA1mg / L NAA0.1mg / L, Tong Jiang lilies and Whitehaven co callus proliferation and differentiation of the best medium for MS 6-BA0 .5 mg / L NAA0.1mg / L. (3) colchicine and oryzalin immersion method for lily polyploid induction studies showed that: In order to maximize the survival rate of plants mutagenesis, 0.02% colchicine plus 2% DMSO, processing 24h, 0.006% Oryzalin plus 2% of DMSO, processing 24h, more suitable for the lily chromosome doubling the concentration and time. Mutation rates were: 'Siberia '22 .22%, 18.89%, 28.89%, Qingdao lily, 18.33%, 15.56% Tongjiang lily, 11.11%, 11.11% Lilium, 8.89%. Oryzalin mutation rate is relatively low, but also less toxic to plants, plant mortality is low after mutagenesis, alternative colchicine-induced polyploid lily generated. Polyploidy in apparent morphology were associated with normal plants are quite different, showing a huge thick, can be used as a basis for identification of early polyploid. Flow cytometry analysis and identification of polyploid test material required less speed, more accurate test for the identification of polyploid. Through the 'Siberia', Qingdao lily, Lilium, lily for induction Tongjiang and identified, it was confirmed that polyploid strains.
|