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Chinese narcissus (Narcissus tazetta var.chinensis Roem) is Amaryllidaceae perennial ornamental plants, flowering during the Spring Festival, is one of China's top ten traditional flowers, with high economic and ornamental value. As it is Autotriploid, there is a great traditional cross breeding difficulties; resources are scarce and it is wild, a serious pest, the growth cycle is long, high temperature requirements grow and blossom, resulting in an increasingly competitive world it flowers competition in the industry challenges. Therefore, this study by RT-PCR, cloning and China daffodils developmental gene NTLEAFY and lectin genes NTA, the use of bioinformatics methods for its nucleotide sequence encoding the amino acid sequence analysis, and carried out a genetic transformation study, the following conclusions: 1, NTLEAFY gene length of 1340 bp, containing the complete coding frame 1137 bp, encoding 378 amino acids, the amino acid sequence encoded pI and molecular weight, respectively 8.00 and 42 274.65 Da. Blast through the NCBI website analysis, NTLEAFY gene and Daffodils, hyacinth and other LFY homologous genes from different species consistency of 76% or more; NTLEAFY genes encoding proteins with Daffodils, hyacinth, Arabidopsis LFY homologous genes encoding The consistency of more than 57% protein. Homology analysis, NTLEAFY transcription factor encoded by amino acids have a basic structure, NTLEAFY LFY protein proteins in other species having a variable region and a C-terminal conserved higher. Protein structure prediction that China Narcissus NTLEAFY proteins in Arabidopsis LFY proteins are very similar in structure. Using real-time quantitative PCR methods, use as a reference gene GAPDH, different organizations on Chinese narcissus, flower development in different periods and in different organs NTLEAFY gene transcription level expression were detected. The results showed that, NTLEAFY genes in reproductive organs and vegetative organs were expressed, NTLEAFY gene expression is high in bud, flowers subsequent expression of the expression levels of buds only 1/8; NTLEAFY gene daffodils in China The higher expression of stamens, while pistil, flower and vice Guanzhong expression levels are low, indicating that its Chinese narcissus vegetative and reproductive growth has a certain role in regulating. This study constructed NTLEAFY genes of plants overexpressing the carrier, through the floral dip method into Arabidopsis and obtained transgenic plants, however, the transgenic plants did not early flowering, and transgenic plants dwarf, leaf malformation, leaf and apical inflorescence with flowers shape, and there have been five petal flower. Preliminary indication NTLEAFY genes involved in flowering transgenic plants and flowers in rats. 2, this study using RT-PCR method, from the Chinese daffodil bud lectin gene was cloned NTA, the use of bioinformatics methods for its nucleotide sequence encoding the amino acid sequence of its protein structure analysis and prediction. The results show that the resulting NTA gene is 698 bp, containing a complete open reading frame 519 bp. This gene encodes a protein of 172 amino acid precursor protein lectin, the precursor protein isoelectric point and molecular weight, respectively 5.84 and 18 615.19Da. Sequence analysis showed that the sequence with the other NTA Chinese Narcissus lectin gene monocots reached more than 82% similarity, NTA gene encoding the protein and other monocots lectin protein in more than 77% homology. Protein structure prediction that China Narcissus Daffodils lectin protein lectin protein is very similar in structure. Protein encoded by this gene and protein structure simulation analysis shows that the protein contains three specific functional domains and alpha-D-mannose binding surface (QXDXNXVXY). In this study, the plant was constructed over NTA gene expression vectors, transformed via the floral dip method and obtained transgenic Arabidopsis plants, transgenic plants flowers compared with the control, no significant difference, stem straight, collateral less, and obviously stems thick, leaves large, long and large pods.
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