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In this paper, a small stab Hericium [H.caput-medusae (Bull.: Fr.) Pers.] Submerged fermentation extract as raw material, the extracted oligosaccharides (HPY) for separation and purification, physicochemical properties, stability, biological activity conducted a preliminary study, the following conclusions: 1 by ethanol precipitation method HPY pretreatment, raw sugar yield was 15.5%, followed by acid treatment, repeated freezing and thawing, filter membrane, through the Q-Sepharose FF column chromatography, first select three elution ionic strength on the polysaccharide, the results show that the majority of raw sugar without protein is neutral oligosaccharides, and applying anion macroporous resin adsorption series, in optimum conditions, specifically one of oligosaccharides were separated on protein-free, sugar loss was 50.5%, so the total yield 7.89% polysaccharides, such a small thorn Hericium extract submerged fermentation process for the preparation of oligosaccharides oligosaccharides fungal drug development provide sufficient raw materials. 2 by phenol - sulfuric acid method spines Hericium submerged fermentation extract purified oligosaccharide (HPY-1) of the total sugar content of 97.59%, and its determination method is stable and reproducible, high thermal stability. Method and Superdex 30p manager gel column chromatographic analysis, the conclusion HPY-1 single component neutral non-reducing oligosaccharide, ultraviolet absorption spectrum shows no HPY-1 nucleic acid, protein absorption, infrared spectral analysis HPY-1 show a characteristic peak of carbohydrate, and the glycosidic bond of β-type configuration. Intrinsic viscosity [η] = 45. HPY-1 by acetylation, GC analysis to determine if the group consisting of monosaccharide glucose. 3 In the antioxidant study, in turn HPY HPY-1 and reducing power, superoxide anion radicals and hydroxyl radical scavenging a comparative analysis: HPY reducing power was enhanced with increasing concentration. When at a concentration of 10mg/ml, HPY-1 reducing power reached 1.202; at the same concentration, HPY-1 reducing power significantly better than HPY. The pyrogallol measured by superoxide anion scavenging rate: HPY-1.3 (33.6%)> HPY-1.2 (25.9%)> HPY3 (19.3%)> HPY2 (15.6%)> HPY-1.1 (11.5%) > HPY1 (7.9%); Similarly, the same concentration, HPY-1 on superoxide anion scavenging ability was significantly better than HPY; same sample, with the sample concentration increases, the superoxide anion scavenging rates are increasing, at a concentration of When 0.4mg/ml, HPY-1 removal rate of 33.6%. Using experimentally measured scavenging hydroxyl radical scavenging rate: HPY-1.3 (46.72%)> HPY3 (31.52%)> HPY-1.2 (29.48%)> HPY2 (17.93%)> HPY-1.1 (7.51%) > HPY1 (3.58%). 4 using the MTT assay Hela and SMMC-7721 tumor cells in vitro IC 50 , the results show that, HPY for 24h on the experiment with a strong inhibition of tumor cell proliferation, the highest inhibition rate reached 70% above. Can be found, HPY-1 on Hela and the proliferation of SMMC-7721 cells significantly inhibited, and presents a good dose - effect relationship.
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