|
Abstract As food safety and health consciousness enhancement, people pay more attention tothe analysis of pesticide residues in food. Macroscopically speaking, the analysis ofpesticide residues include collection, pretreatment, extraction, purification, concentration,detection and analysis. Sample pretreatment technology was a key part of the residuesanalysis. Accordingly, it required a high sensitivity, low detection limit, accuracy andgood selectivity. Pesticide residues analysis was more complex, the direct determination of traceanalytes in the complex samples by modern apparatus was very difficult. So someappropriate separation and preconception methods were necessary. In the analysis ofpesticide residues, common interferences include lipid, pigment, amino acid derivatives,carbohydrates, lignin and terpene. In the whole sample analysis process, the time ofsample pretreatment technology accounted for two-thirds in the entire analysis time.Therefore, a simple, rapid, efficiency and solvent-free extraction as a promisingtechnique for sample preparation and pretreatment has become one of the most importantresearch areas in modern analytical chemistry and attracted much attention recently. In this study, new methods based on DSPE and DLLME-SFO combined with highperformance liquid chromatography had been developed for the determination offungicides in complex samples. The paper focused on the application of green solvents inorder to decrease environmental pollutions. The major contents are described as follows: 1. This method based on DSPE combined with HPLC has been established. In thisextraction technique, acetontrile and PSA were selected as extraction and sorbentsolvents, respectively. DSPE operational parameters were studied and optimized, and theoperation conditions were applied to the fruit juice samples to examine the application ofthis method. 2. The pretreatment method of DLLME-SFO combined with HPLC was used fordetermination of two fungicides in enviromental water. In this extraction technique,1-undecanol and methanol were selected as extraction and dispersive solvents,respectively. This paper investigated the corresponding extraction factors and determination conditions. On that basis, this paper was applied for the analysis of the fungicides in real samples.3. This paper combined the purification ability of DSPE with the high extraction efficiency of DLLME-SFO in sample preparation and developed a new technique to determinate the two fungicides in fruit juice. This method will extend its application to more complex matrix samples. A series of parameters influencing extraction were investigated. On that basis, this paper was applied for the analysis of the fungicides in the fruit juice.
|