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Authentication of Germplasm Resource of Rhizoma Pinelliae and the Effect of Processing Method on Its Quality

Author: ChenGang
Tutor: ChenKeLi
School: Hubei College of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Pinellia Isoelectric focusing Gas Chromatography High Performance Liquid Chromatography Sedative and hypnotic
CLC: S567.239
Type: Master's thesis
Year: 2009
Downloads: 138
Quote: 0
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Abstract

Breit, Araceae Pinellia Pinellia ternata (Thunb.) Breit. Drying tubers, dampness phlegm, Jiangni antiemetic, Xiaopi the the Sanjie effect. According to statistics in the frequency of use of 558 kinds of traditional Chinese medicine prescription, Pinellia ranks 22. Its wide range of pharmacological effects and is blindly important of the traditional Chinese medicine. Pinellia content of each component, the active ingredient is not clear, the huge difference in quality due to different methods of processing Pinellia has not attracted enough attention, a long time Pinellia processing there is no uniform standard of science, but also because of the kinds of there is a big difference in quality of resources, place of origin, different harvesting time, the quality of Rhizoma seriously affect its clinical efficacy. In this paper, collected from all over the dry, fresh goods Pinellia as research subjects, respectively, using isoelectric focusing electrophoresis, gas chromatography, reversed-phase high performance liquid chromatography of these samples were measured and analyzed to explore Pinellia germplasm its chemical composition and the different processing methods, to lay the foundation for the the Pinellia germplasm resource identification and quality standards research. Germplasm material from different localities, maturity and germination period Pinellia isoelectric focusing (IEF) analysis. The technique is to form a relatively smooth pH gradient by addition of ampholytes, and stop at the isoelectric point of the sample protein electrophoresis. All kinds of Chinese herbal medicines containing different protein components can be applied IEF technology, it is the quality of analysis and identification. Ordinary electrophoresis equipment to complete in order to reduce the expensive reagent dosage IEF electrophoresis, isoelectric focusing of the application of the improved good results and good reproducibility. Experimental results show that: the different regions of Pinellia is mature protein IEF electrophoresis spectrum is the same, but the growing resources of the different regions in the germination period Pinellia the IEF protein electrophoresis spectrum there is a significant difference between; same area (such as Jingzhou wild Pinellia) germination period a tuber the IEF protein electrophoresis spectrum is the same. Therefore, we can consider the germination period of Pinellia to identify different regions or different the Pinellia varieties of germplasm resources. This article by gas chromatography on the the sterol components contained in the processing of a variety of the various regions using different methods Pinellia sample analyzed Determination results show that: by 70 ° C and baking 2h and then to low temperature drying EXi Pinellia The sample, which contains five unique sterol composition is very rich and stable, and five each sample component peaks high similarity. And without proper high temperature processing, drying pinellia samples, or direct natural dried the Pinellia samples, sterols ingredients should peak or almost difficult to detect the corresponding component was not detected. We also carried out in a germination period Pinellia samples tested, completely did not detect the Pinellia specific sterol component. Our analysis, the Pinellia some enzymes involved in Pinellia sterols ingredients metabolism, germination period, the enzyme is especially active substances rapid metabolism, so it is difficult to be detected sterols ingredients. Low temperature drying and dried Rhizoma polyphenol oxidases unspoiled, just part of the activity is inhibited due to the drying process, and once the temperature rises, the air become damp, which contained enzymes became active. Without the appropriate high temperature treatment to kill the enzyme Breit, in the role of polyphenol oxidase browning or even rot. Some people prefer to prevent this phenomenon, Pinellia often joined a preservative called sodium metabisulfite (a kind of substances that cause cancer), and seriously affect the quality of Rhizoma. In this paper, the the Pharmacopoeia conventional method to view results found: some pinellia sample does contain sodium metabisulfite. The low temperature drying and the Rhizoma dried ingredients easily changed, highly perishable the reasons contained polyphenol oxidase destruction. The results of this study reveal, the Pinellia processing has a very close relationship to its quality and storage. The results of the above analysis, the paper also confirmed by the analysis of water-soluble components in Pinellia. By reversed-phase high-performance liquid chromatography with methanol - water system as mobile phase, detection wavelength of 268nm, column temperature was 30 ℃, the flow rate of 1.0 mL x min ^{-1 the Pinellia sample processing different were detected. Experimental results show that: without proper high temperature to kill Pinellia and enzyme treatment in the germination of fresh goods Pinellia at 70 ℃ bake 2h and then low-temperature drying of Pinellia just excavation the fresh goods pinellia compared a water-soluble chemical composition is significantly reduced. And when the correct sample and reference substance, also can be used the established HPLC chromatography on the Pinellia water-soluble fingerprints for further analysis. Finally, the use of subthreshold and suprathreshold dose of pentobarbital sodium in mice sleep, and investigated by the appropriate high-temperature processing the western Hubei Pinellia sedative activity intensity and the active site. The results show that: the Pinellia alcohol extract of ethyl acetate extract total alcohol extract and alcohol extract of the n-butanol parts compared to a stronger sedative and hypnotic effects, half-Xia Zhenjing hypnosis effective component should exist in their alcohol extract of the ethyl acetate extract.}

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