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An Optimized Screening, Identification of Armillaria Mellea Complex and Influence on Yield of Gastrodia Elata f. Glauca

Author: JiNing
Tutor: LiYu
School: Jilin Agricultural University
Course: Plant Pathology
Keywords: A. mellea Ukrainian Gastrodia Filter Yield Species
CLC: S567.39
Type: Master's thesis
Year: 2008
Downloads: 216
Quote: 0
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Abstract


Armillaria mellea is a wide host range, pathogenicity, an important pathogen of worldwide distribution. A. mellea has many distinctive features, it can not only be parasitic, but also saprophytic, but also symbiotic with Tianma and Polyporus. Armillaria mellea is a higher economic value of edible and medicinal fungi. Tianma is a valuable medicinal plants to treat pediatric convulsion, stroke, hand, foot and fail to materialize, neuralgia and other diseases of the role, especially in recent years research found Gastrodia anti-Alzheimer. Tianma is a plant that has no root, leaf differentiation, therefore it can not be done by root absorption of nutrients, and can not carry out photosynthesis. The Gastrodia growth and development must rely on the Armillaria mellea to provide most of the nutrition. Armillaria mellea strains nurture improve the yield and quality of Gastrodia an important role in the production of quality Tianma. Production of Jilin Province the Gastrodia cultivation in red gastrodin and Wu Gastrodia. Wu Gastrodia especially good shape, good quality medicinal dry discount rate, and thus considerable economic value. This experiment were isolated and purified wild honey collected from the main producing areas of Gastrodia Changbai Mountain region mellea and Gastrodia, a total of 14 strains of Armillaria screened excellent traits of five strains, and partners planted the Changbai Mountain Ukrainian Gastrodia test. Ultimately determine suitable in the excellent honey of the Changbai Mountain Ukrainian Gastrodia growth mellea strains, and the identification of its species. This study completed the following work, and the corresponding conclusions: 1 A. mellea medium screening. Strains on different media culture Ar-11 Determination of characteristics observed and different periods of biomass yield, the following conclusions: A. mellea peptone medium rhizomorph germination time, full bottle shorter time rhizomorph developed, high biomass yield. Therefore, for the purpose of rapid culture strains of Armillaria, you can choose the peptone medium. Strain Ar-11 cultured on PDA medium, 25 ℃ culture 25d biomass yield, indicating that A. mellea growth on PDA medium stable, slow aging PDA medium as A. mellea strains save with. 2 A. mellea high efficient strains. Armillaria mellea strains 14 solid culture characteristics, characteristics of liquid culture, preliminary screening five excellent Armillaria mellea (strain Ar-04 Ar-07 Ar-11 Ar-13, AT-14 ), Gastrodia companion planting test, further testing of its excellent features. \The the 3 A. mellea Wu Gastrodia companion planting trials, research Armillaria mellea strains Wu Gastrodia production. 5 strains were compared with the control test, the test results show that: the strain of Ar-04 in the two test methods, there was a significant (P lt; 0.05) differences with the control. Strain Ar-11 in imitation of wild cultivation experiment with the control exist significant differences in sex, in box planted test the difference was not significant. 4 Armillaria mellea strains Ar-04 Ar-11 species identified. Strain Ar-04, Ar-11, single spore isolates, single spore isolates used to be tested in 2% MEA medium for mating with the NE and five kinds of the test strain, and ultimately determine the strain an Ar-04, Ar-11 CBS B, Gaul A. mellea.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Fungus > Other
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