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Skullcap (Scutellaria baicalensis Georgi) years for the Labiatae herb root used as medicine for our common bulk herbs, as China continues to develop the process of modernization of Chinese medicine, herbs skullcap demand increased year by year, decimated wildlife resources, has been unable to meet market demand. At present, the cultivation skullcap Scutellaria natural herbs has become the main source, but about cultivation and physiological characteristics of Scutellaria few studies on skullcap under stress conditions protective enzymes and their related physiological changes have not been reported. Therefore, to carry out the skullcap under stress conditions physiological and biochemical characteristics of research, to guide skullcap cultivation and medicinal raw materials to achieve the security of supply and stability of quality control is of great significance. In this paper, skullcap (Scutellaria baicalensis Georgi) as experimental materials to study skullcap protective enzymes (SOD, CAT, POD) activity, malondialdehyde (MDA) content, soluble protein content, soluble sugar content and other physiological indicators of changes in the law, stating moisture, light, soil and other ecological factors on Chinese skullcap growth and development, and explore under stress conditions protective enzymes and their associated physiological variation, skullcap adapt to the environment of the mechanism to determine the skullcap optimum growing environment for skullcap cultivated planting scale production of high-quality ingredients and provide a scientific basis. Periodic sampling test period skullcap, Scutellaria determined by conventional methods protective enzyme activities and soluble protein, soluble sugar content and other indicators. The main results are as follows: In the environmental conditions on seed germination tests, through the skullcap seed weight and germination rate, the results showed that: in different habitats, skullcap seed weight is different Taonan skullcap seed weight, but its right skullcap seed germination rate did not affect the three places skullcap seed germination same trend, germination rates differ little; seed storage for a long time, germination rate decreased, storage over 2 years seed should not do the production of seed; skullcap soaking the suitable water temperature is 40-50 ℃, soaking time 6h-12h, suitable temperature for germination of 20-25 ℃, the light environment skullcap germination rate significantly increased. Skullcap seed germination tests showed protective enzymes, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activity and skullcap seed germination showed significant or highly significant positive correlation. Skullcap seed germination process, several protective enzymes are then new seed gt; 1 Year seed gt; 2 years seeds, protective enzymes closely with skullcap seed resistance, skullcap seeds increased with storage time, seeds resistance reduced. Environmental conditions on the cultivation skullcap protective enzymes and related physiological indices trials, through the skullcap protective enzyme activities and their associated physiological studies, the results showed that: strong light can increase the skullcap superoxide dismutase (SOD) , catalase (CAT) activity, soluble protein content and lower malondialdehyde (MDA) content of soluble sugar content had little effect; water and moisture frequency experiment shows moderate drought can increase a year old skullcap protective activity, increased soluble protein and soluble sugar content, and enhance the resilience of plants, while the two-year-old skullcap, protective enzymes and their associated physiological aridity increases with decrease; in the soil test, soil treatment Taonan SOD, CAT and POD activity was the highest, followed by Changchun soil treatment, soil treatment Riverside minimum. Cultivation skullcap tests showed physiological characteristics of different periods, 1,2 years old skullcap smaller differences soluble sugar content, CAT together early in the reproductive activity of POD slightly higher than the flat intermediate changes, post high, MDA content and the POD activity was negatively correlated. 1 year old skullcap different leaf soluble protein content decreased sequentially from top to bottom, 2 years old skullcap soluble protein content is lower leaves highest, followed by the upper, middle lowest. 1,2 years old skullcap different leaf soluble sugar content changes more consistent, the lower was the highest, followed by the middle and upper minimum. On the whole plant, the one-year-old skullcap soluble protein and soluble sugar content higher than 2 years old skullcap. Different ecological zones skullcap physiological characteristics tests showed that Taonan ecological zones soluble protein and soluble sugar content is high, followed by ecological zone in Changchun, Riverside lowest ecological zone.
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