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Is arid and semi-arid areas of Xinjiang, 31.1% of the existing arable land contaminated by saline hazards, while 80% of the arable land salinization of secondary salinization, and seriously affected the germination and emergence of cotton and tomato seeds as a constraint to cotton in Xinjiang The major factor in the development and tomato. In order to solve the problems of the region, the paper in microbial cotton, tomato Promotion and Disease based on previous work, the study five mixed culture of bacteria, aims to provide the scientific and theoretical basis for large-scale production and application of composite inocula. Which two bacteria for salt tolerance promoting bacteria to improve crop tolerance to saline soil environment; 3 bacteria living anti-bacterial, able to control crop seedling diseases. The experiment contains the following five aspects: 1, strain identification and antagonistic symbiosis research: the use of strains of morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence homology with salt-tolerant growth promotion and disease prevention role of bacterial Rs-198 , SL-13, SL-14 and SL-44 were identified. Preliminary identification results showed that the RS-198 Pseudomonas fluorescens (Pseudomonas fluorescens), SL-13, SL-14 and SL-44 are atrophy of Bacillus (Bacillus atrophaeus); RS-5, Rs-198, SL -13, the interaction between the SL-14 and SL-44 five bacterial strains of the combination of two test results show that the Rs-5 antagonists SL-13; RS-198 antagonistic SL-13, SL-14; SL-13 antagonistic SL -14; SL-44 antagonistic SL-13, SL-14; remaining in any combination can be symbiotic. 2 mixed culture conditions study: the the selected symbiotic salt tolerance promoting bacteria Rs-198 and Rs-5 combinations mixed culture. First, according to the different temperatures and initial pH of the medium, the growth of strain Rs-5 and Rs-198, mixed culture temperature to 28 ° C and initial pH of 6.5; Secondly, through analysis of Rs-198 and Rs- 5 in different proportions mixed mode, the residual amount of glucose, the biomass generation and changes in pH, revealing the strains have a synergistic growth role in determining the concentration of optimum mixing ratio of 106:106. 3, medium optimization: RS-198 and Rs-5 per ml concentration of viable count 106:106 mixing ratio of orthogonal experimental optimization mixed medium: glucose 25g / L; peptone 35 g / L; the yeast powder 15g / L; Na2 HPO4 · 12H2O10g / L; MgSO4 · 7H2O1.2g / L; KH2PO4 3.63g / L. 4, mixed anti-efficiency test: pot experiment showed that using mixed inoculant can improve the germination rate of 22.1%, field experiments showed that the mixed inoculant enables 10.05% increase in yield per mu, and then verify the role of the control efficiency of the mixed inocula. 5, the shelf life of the mixed inoculant: made of different bacterial agent placed in a dark place at room temperature to save every month to detect the number of viable cells. The results showed that after 5 months of storage, the Rs-5, SL-14, the viable cell concentration of the BCL-8 water inocula were increased 1.63,1.72,1.20 logarithmic; Rs-198, SL-13, SL the -44 water inocula of viable bacteria concentration decreased 0.56,0.55,0.28 logarithmic.
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