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Detection of Seed-borne Viruses in Soybean and Common Bean Seeds Conserved in the National Crop GeneBank

Author: HanJunLi
Tutor: WangXiaoMing；GuoQingYuan
School: Xinjiang Agricultural University
Course: Plant Pathology
Keywords: Soybean Kidney bean Stock germplasm Seed-borne virus Detect
CLC: S432.41
Type: Master's thesis
Year: 2009
Downloads: 46
Quote: 1
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Abstract


Leguminous crops susceptible to a variety of plant virus infection with a virus germplasm quality to be significantly affected , but also seriously affect the safety of germplasm preservation . In this study, the National Germplasm interim library 300 soybeans, 100 Bean Germplasm materials, serological and molecular biology methods were used to detect the types of seeds, seed-borne virus . Through the storage of germplasm carry the detection of the virus type and quantity of different geographical sources of germplasm infected Features information to infer the spread of the virus situation in the production of seed multiplication , seed-borne virus on the safe keeping of germplasm and seed security threats, and then explore the control of the storage of germplasm carrying seed-borne virus prevention approach , inventory germplasm seed-borne virus and germplasm safe keeping technology to lay a good foundation for the future in-depth study . The main findings are as follows : 1 . Development based on cucumber mosaic virus CP gene sequences the detection CMV specific primers CMV-CP-491F/CMV-CP-491R of : soybean seeds of cucumber mosaic virus can detect , and for disease diagnosis and pathogen identification . Built for the qualitative detection of soybean seeds carry SMV , the CMV of the composite RT - PCR technology, can effectively use the technology stock germplasm , the entry of soybean germplasm and field seed production and health quality assessment , reducing the detection time, reducing testing costs . 2 types of seeds, seed-borne virus were detected. The results showed that : detected in soybean germplasm of soybean mosaic virus ( SMV ) , cucumber mosaic virus ( CMV ) , alfalfa mosaic virus ( AMV ) three viruses , the positive rate was 24.67% (74 ) 12.67% (38 ) and 4.67% (14 ) . Soybean germplasm of soybean mosaic virus and cucumber mosaic virus , soybean mosaic virus and alfalfa mosaic virus complex infection . Bean Germplasm detected bean common mosaic virus ( BCMV ) - positive material of 92 the germplasm infection rate of up to 92% . These provide the basis for information security crop germplasm resources preservation and health applications . 3 the breeding germplasm health conditions , will in the future take relevant measures to improve the quality of beans into the the national genebank save the germplasm to help.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant diseases and their prevention > Transgression ( pass ) an infectious agent harmful > Virus
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