Dissertation > Excellent graduate degree dissertation topics show

Immobilization of Glucose Isomerase and Its Performance Study

Author: YuHaiTao
Tutor: GuoYangLong
School: East China University of Science and Technology
Course: Industrial Catalysis
Keywords: polymer support epoxy groups glucose isomerase covalent immobilization operation stability
CLC: TQ925
Type: Master's thesis
Year: 2011
Downloads: 215
Quote: 0
Read: Download Dissertation

Abstract


The polymer carrier GAMM with epoxy groups was synthesized by the inverse suspension polymerization, in which Tween-20 and Span-60 were used as the complex dispersants, and N, N’-methylene-bis (acryla-mide) (MBAA) as crosslinking agent, and ally glycidol ether (AGE) and glycidyl methacrylate (GMA) were used as reactive monomers. The carrier was used to immobilize glucose isomerase (GI), in which effects of immobilization conditions and isomerization reaction conditions on the activity of immobilized GI and the operating performance of immobilized GI were investigated.The optimum immobilization conditions were as follows:the loading amount of free GI was 0.3 mL per gram carrier, the immobilization temperature was 25℃, and the immobilization time was 24 h; The optimum reaction pH values of free and immobilized GIs were both 7.5. Immobilized GI was more stable at pH value of 5-7.5 and had wider application pH range, The optimum reaction temperature was 65℃. As for free and immobilized GIs, the kinetic parameters were calculated as 0.36 and 1.16 mol/L for Km, and 1.3×10-3 and 1.1×10-3 mol/L·min for Vmax, respectively. The maximum activity of immobilized GI was 450 U/g (wet). The relative activity of immobilized GI was about 90% after recycled for 18 times and retained 97%of its initial activity after stored at 4℃for six weeks, immobilized GI had better thermal stability than free GI below 80℃.

Related Dissertations

  1. Study on Screening of Glucose Isomerase Producing Strain and Enzyme Properties,TQ925
  2. The Clinical Role of Glucose-6 Phosphate Isomerase in Diagnosis and Curative Effect Evaluation of Rheumatoid Arthritis and Effect of Compound Salvia M Iltiorrhizal Injection on Glucose-6 Phosphate Isomerase,R259
  3. Expression and Characterization of a Thermostable Glucose Isomerase from the Hyperthermophile, Thermotoga Maritima,Q93
  4. Study on Population Genetics of Phytophthora Infestans Strains from Fujian Province,S435.32
  5. Glycolysis and the Involved Enzymes in Bacillus Sphaericus,Q93
  6. Research on Urban Sewage Sludge Treatment with Themophilic Aerobic-mesophilic Anaerobic Digestion,X703
  7. Study of Liquid Phase Oxidation Based on Modified Polyoxometallates,O643.36
  8. Synthesis and Covalent Immobilization of Naphthalimide and Other Derivatives as New-type Fluorescence Carriers for Optical Fiber Chemical Sensor Preparation,TP212
  9. High specific speed Francis turbine operating at low load stability,TK733.1
  10. Research on Stability of Revolving Floating Cranes,U674.3
  11. The Stability Study of the Slope by the Complicated Rock and Soil Including Underground Empty District,TU43
  12. Analysis and Computation on Limit of Leading Phase Operation of Powerformer,TM31
  13. Immobilized on electronic media to build electrochemical biosensor research and application,TP212.3
  14. The Novel Immobilization Methods of Indicators and Their Applications in Preparation of Fluorescence Sensors,TP212.1
  15. Based study of the sync separation analysis of multi-channel mode filter detection photochemical sensor,TP212.2
  16. Novel Fluorescence Sensors Based on Covalent Immobilization under UV Radiation of Fluorescent Carrier,TP212
  17. Fluorescent Chemosensing Technology for Determination of Heavy Metal Ions Based on Organic Carriers,O657.3
  18. Synthesis of New Fluorescence Carriers and Their Application in Fluorescence pH Sensor Preparation,TP212.2
  19. Development of Novel DNA Biosensor Based on Covalent Immobilization,TP212.3
  20. The Study on the PLD Immobilization by Covalent and Enrich Cross-linking Technologies,Q814.2
  21. The Research on Preparation and Performance of Fluorescence Chemical Sensor and Their Application in Environmental Analytical Chemistry,TP212.2

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > Enzyme preparation ( enzyme )
© 2012 www.DissertationTopic.Net  Mobile