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With the development of modern industry , especially the extensive use of fossil fuels , and polycyclic aromatic hydrocarbons in the environment (Polycyclic Aromatic Hydrocarbons, increasingly referred PAHs) content . PAHs have teratogenic , carcinogenic , mutagenic characteristics , seriously endangering the health of plants , animals and humans . Therefore , further study clearly flora and fauna and how to respond to PAHs Stress can provide a theoretical basis for its prevention and treatment . The plant can absorb the atmosphere of PAHs in the soil and can produce some of the stress response . However , the molecular mechanisms of plant responses to PAHs Stress remains unclear. The present study is based on known PAHs Philippines Stress Arabidopsis differentially expressed proteins , search the TAIR database encoding gene from gene activation and inactivation live are two ways to understand these genes in response to the the PAHs Philippine stress possible role, made ??the following main results : building Arabidopsis response PAHs phenanthrene stress 9 differentially expressed protein over-expression of the corresponding gene (At4g11010, At2g36460, At3g52960, At4g38740, At2g44060, At1g11860, At5g17920, At1g50480, At1g70890) carrier , with Agrobacterium-mediated transformation of Arabidopsis thaliana , by semi-quantitative PCR validation screening to the over-expression of the four genes (At4g11010, At2g36460, At3g52960, At4g38740) plants . At4g11010 overexpression of plant root growth of phenanthrene Stress is more sensitive , significantly shorter than the wild-type plants , but not observed in other plants overexpressing obvious phenotypic changes . Further to the T-DNA insertion inactivation mutant for the material , state of functional inactivation of these genes , phenotypic changes in plants phenanthrene stress , but also obvious phenotypic differences were not observed . The results for the understanding of these genes in Arabidopsis response PAHs Stress functions provide a scientific basis , shows the complexity of the molecular mechanisms of Arabidopsis response PAHs phenanthrene stress .
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