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New bat BOULE gene cloning and identification of isomers

Author: ZuoXueGuo
Tutor: ZhangShuYi
School: East China Normal University
Course: Ecology
Keywords: BOULE gene Chiroptera Sperm storage Transcription isomers
CLC: Q78
Type: Master's thesis
Year: 2009
Downloads: 50
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Abstract


Bats are the second largest category of mammals, it has a lot of special reproductive phenomena . For example , in order to adapt to harsh living environment and enable future generations to remain prosperous, many bats have the ability to store sperm and sperm storage and sperm quantity, quality , time, three indicators are closely related to sperm motility , studies show that many bats have formed an effective sperm storage reproductive strategies . Spermatogenesis is a complex process and are subject to strict regulation of many genes . The available data indicate that twine gene can initiate meiosis to produce sperm , and twine BOULE gene expression by strict control, so most scholars believe BOULE gene in the control males produce gametes plays an important role in regulating . In order to determine whether to participate and how BOULE gene involved in sperm storage bat , we cloned and analyzed , including large Myotis , greater horseshoe bat, big brown tongued fruit bats and fruit bats , including four kinds of bats BOULE gene coding sequence , of which the first two bats with sperm storage capacity , the latter two bats do not have this capability. The results showed that at least two of the bat BOULE gene transcripts isomers (a and b), where a -isomer and other mammals BOULE genetically similar , and b is the first isomer found in bats . Since the b- type isomer bat exon deletion resulting in vitro translation reading frame and early termination of the length difference , which leads to some loss of the ribbon . We downloaded from GenBank database with a bat -isomer of 15 mammalian homologue BOULE gene sequence selection pressure . Analysis showed that the BOULE gene in mammalian evolution by the strong role of purifying selection , bats and other mammals are also no significant selective pressure difference . Therefore , BOULE gene transcription may be varied by changing the expression levels of different isoforms involved in the regulation of sperm storage bat . This is the first cloned and characterized the bat BOULE gene multiple isoforms , combined with evolutionary analysis showed that bats during spermatogenesis may BOULE there is a new mechanism of gene regulation .

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