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Nanomaterials on phenothiazine drugs with DNA research impact

Author: ChenZhuQing
Tutor: DingYuanHua；GuoRong
School: Yangzhou University
Course: Physical and chemical
Keywords: Gold nanoparticles Thionine Anionic surfactants Interaction Methylene blue Fluorescence Spectrometry Dimer Small Size Electrostatic Fluorescence Polarization
CLC: TB383.1
Type: Master's thesis
Year: 2009
Downloads: 74
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Abstract

1 Thionine and anionic surfactant SDS interaction study, UV spectroscopy, fluorescence spectroscopy study Yin surfactant sodium dodecyl sulfate (SDS) and thionine interactions between molecules and studied hydrophilic groups of different chain lengths and of anionic surfactants. Found on SDS less than 8.0 × 10-3 mol / L before the increase as the concentration of thionine significantly from a monomer molecule to the H?-And J? Homodimer transition and the hydrophobic anionic surfactant The longer the chain, or has a flat rigid ring, the more easily induced thionine molecule changes from monomer to dimer. In SDS concentrations greater than 8.0 × 10-3 mol / L, the thionine molecule consists of dimer to monomer transition. Fluorescence polarization, NMR studies show that the anionic surfactant SDS is smaller than its critical micelle concentration (cmc) before thionine monomer molecules are mainly located in the hydrophobic chain of SDS; when SDS micelles after thionine molecules increases solution to the micelle palisade layer. Isothermal titration calorimetry results showed that the SDS concentration is less than 8.0 × 10-3 mol / L when, SDS and thionine molecular hydrophobic association and thionine dimerization is a major, system performance is exothermic; when the SDS concentration is greater than 8.0 × 10-3 mol / L time, SDS micelle dissociation based, system performance of the endothermic direction; when cmc concentration obtained after SDS, SDS interaction with saturated thionine, system dominated by the dilution effect. 2 anionic surfactant SDS on thionine with DNA regulation by UV spectroscopy, fluorescence spectroscopy to study the SDS on thionine? DNA interaction effect was found of SDS makes the DNA on the association thionine transition from dimer to monomer, and the higher the concentration of SDS, the stronger the effect this change, indicating that SDS on thionine competitive associative make thionine from DNA transfer to SDS aggregates. By fluorescence polarization was found that with increasing concentration of SDS, thionine rotational diffusion velocity increases, and gradually reached equilibrium, indicating thionine more closely into the environment, verify the results of the spectroscopy. Circular dichroism results show that the intercalation of SDS makes a Thionine conformation of DNA from B-to-A transition, indicating that the SDS hydrophobic chain and DNA base pairs exist between the hydrophobic effect, resulting in DNA double helix structure has changed. 3 nanoparticle interaction with thionine studied by UV spectroscopy, fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy, isothermal titration calorimetry, and infrared spectroscopy method to study the thionine at 18 nm and 5 nm in two sizes of gold nanoparticles on the association behavior. Study found that small gold nanoparticles can thionine monomer molecule peaks gradually disappeared, and dimer peaks significantly enhanced. Due to the small size of the gold nanoparticles on a larger surface area per unit volume, the effective absorption area increases, making it easier to make the association on its surface thionine monomer molecules to H? Homodimer change. Small size relative to the large size of gold nanoparticles in terms of thionine quenching is more obvious, and this is the dynamic and static quenching coexist. TEM results showed that adding thionine, the small size of gold nanoparticles formed a large gold? Thionine clusters, and the results measured by DLS gold nanoparticles? Thionine nanocluster size of 45 mm. For 18 nm and nanoparticles of 5 nm, the surface area per unit volume of 0.0597 and 0.2148, respectively. Particle surface area per unit volume greater thionine molecules nanoparticles greater binding free energy. The study will help to understand the organic dyes and metal nanoparticles interaction between the functional organic? Inorganic nanocomposite design provides an important theoretical foundation. 4 of Methylene Blue and Gold Nanoparticles on the regulation of interactions between DNA by UV spectroscopy, fluorescence spectroscopy and fluorescence polarization study the interaction between methylene blue and DNA model, the results show that when the DNA and Asia A molar ratio between the blue lower concentrations, the mode of action between the main role of intercalation; When the mole is relatively high, places the electrostatic interaction. UV spectroscopy, fluorescence spectroscopy and fluorescence polarization results showed that with increasing concentrations of gold nanoparticles, methylene blue to DNA intercalation and electrostatic association is reduced and the impact on the intercalation is more obvious. DLS and TEM results show Mingjinnami resulting particles added methylene? DNA system complexes formed closer and allows displacement out of methylene blue from the DNA. In the course of the action, so that methylene blue gold nanoparticles association of DNA conformation from B-to-A transition. Therefore, we can use gold nanoparticles with DNA regulating the mode of action of methylene blue.

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