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Studies on Different Plantlet Formation Ways of in Vitro Propagation of Nobile-type Dendrobium

Author: ShiJingJing
Tutor: LiQing
School: Beijing Forestry University
Course: Ornamental Plants and Horticulture
Keywords: Nobile Tissue culture Multiple shoots Protocorm
CLC: S682.31
Type: Master's thesis
Year: 2009
Downloads: 195
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Abstract


The nobile watch the high value of the orchid flower market prospects are very bright , and the organizational culture technology is an important way to achieve the nobile factory production , the study of ' Momotaro ' the ( D.Yohkou ' Peach boy' ) , ' Red Star '(D.snowflake' Red star '),' dawn '(D.tomoflake' Akebono ') varieties were established the from multiple shoots breed pathways and protocorm propagation pathways nobile tissue culture system , and tissue culture the various stages of the system to provide a scientific basis for the industrial production of nobile . The test results show that , stem segments the Clustered Shoots nobile breed the best way to start explants with axillary pseudobulb the Best Startup medium : MS 6-BA1.0 mg / L KT 0.5 mg / L NAA 0.1-0.2 mg / L; proliferation medium : MS 6- BA 2.0 mg / L IBA 0.2 mg / L , mashed potatoes, 50-100 g / L . Protocorm propagation pathway induced protocorm directly through the bud tip , the method has not been reported . Best induction medium : MS KT with 2.0 mg / L NAA 0.2 mg / L , dark conditions or add 0.5-1.0g / L of activated carbon can promote induction . Protocorm callus induction materials can be obtained through the induction of callus tissue culture the upper stems segment slice , the best induction medium : MS 2,4- D 0.2 mg / L agar 0.3% ; more injury of transforming the original bulbs suitable medium : MS 6- BA 1.0 mg / L NAA 0.2 mg / L Spring protocorm best proliferation medium : MS TDZ 0.05 mg / L NAA 0.2 mg / L lactalbumin hydrolyzate 0.5-1.0 g / L agar 0.3% ; the protocorm differentiate into seedlings Best medium for : MS TDZ 0.1 mg / L IBA 0.2 mg / L. The plantlets Rooting culture : 1/2MS KT 0.2 mg / L NAA 0.5 mg / L mashed banana 50-100 g / L chlormequat 1.0 mg / L sucrose 15 g / . Plantlet in Transplantation suitable for transplanting matrix as : perlite, sphagnum moss ( 1:1 )

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Other Flowers > Orchids
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