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Construction of High Frequency Regeneration System from Callus of Alfalfa and Mechanism of Main Elements on the Construction

Author: HuangLiZuo
Tutor: AnYuan
School: Shanghai Jiaotong University
Course: Olericulture
Keywords: Alfalfa Callus induction Callus Regeneration System
CLC: S541.9
Type: Master's thesis
Year: 2009
Downloads: 104
Quote: 1
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Abstract


Papers Suntory alfalfa using orthogonal design and single- factor experiment design method of combining Alfalfa callus induction and differentiation process in different types of explants and type of basal medium , as well as different exogenous growth regulator substances on alfalfa callus induction and differentiation , thereby establishing an efficient alfalfa tissue culture regeneration system . The experimental results are as follows : 1 . The best explant of alfalfa callus induction for the next hypocotyl optimum basal medium MS basal medium , the optimal exogenous hormone combination and concentration of 2 mg / L , 4-D 0.5mg / L 6-BA 2.5mg / L KT 0.34 mg / L GA3, callus induction rate was 100% , callus structure is excellent. Alfalfa callus most suitable basal medium for SH basal medium , the optimal combination of exogenous hormones and a concentration of 1 mg / L KT 0.1 mg / L NAA , the best of sucrose , glycine and Mg 2 concentrations of the order of 10 g / L , 4 mg / L and 400 mg / L , the callus rate of 61.82% . Exogenous NAA can reduce callus soluble sugar content , promote callus cells absorb medium Fe 2 and of of Mg 2 < / sup> , improve cell chlorophyll content . Exogenous KT significantly promote callus cells absorbing medium Fe 2 and of of Cu 2 , increased cell chlorophyll content . Exogenous glycine significantly reduce the content of soluble sugar in the differentiation of the callus , increased Cu 2 Fe 2 < / sup> the the Mg 2 < / sup > content trend , thereby increasing the osmotic potential of the callus cells and promote the formation of the genetic material in the cell , increase the rate of cell differentiation of the callus . The outside source Mg 2 concentration can significantly increase the differentiation of callus cells chlorophyll content , which is exogenous Mg 2 the direct cause of the increase in the rate of callus . The results of this study show that the concentration of the hormone optimization SH basal medium , sucrose , glycine and Mg 2 < / sup> can effectively improve the alfalfa callus cells differentiation and morphogenesis .

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CLC: > Agricultural Sciences > Crop > Fodder crops,pasture > Perennial legume > Other
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