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Breeding of strains of marijuana unglued function retting

Author: WangGuiBin
Tutor: GeZuoPing;CaiBaiYan
School: Heilongjiang University
Course: Microbiology
Keywords: Marijuana Unglued bacteria Ferment Response Surface Methodology A hemp rate
CLC: S563.3
Type: Master's thesis
Year: 2009
Downloads: 55
Quote: 2
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Abstract


Marijuana is an important fiber crop , the fiber performance , durability , hygiene and value-added and high fiber raw materials for an environmentally friendly , has a huge potential . The study is mainly by the marijuana microbial retting function Strainfrom , and optimization of enzyme production conditions , so as to establish a complete set of added bacteria retting new degumming process , and to lay the foundation to improve the yield and quality of the marijuana efficiency and fiber degumming . From the Yanshou No. 41 Linen Textile marijuana retting pool retting liquid separation , screening two marijuana unglued function strains the Number for HDDMM02 HDDMG05 . Using morphological, physiological and biochemical tests and phylogenetic analysis of 16S rDNA means of identification to determine the two strains were Bacillus licheniformis Bacillus licheniformis (EU723824) and Escherichia coli bacillus Escherichia coli ( EU723821 ) . Single factor and response surface method to optimize the medium of the two functional bacteria , and to determine the the medium best recipe is the best enzyme production conditions , strain HDDMM02 , Mount taro powder 6 % , peptone, 0.25 % , 0.5% NaCl , MgSO < sub> 4 .7 H 2 O 0.05% , the culture conditions of pH 7 , 2% of the inoculum size , temperature 37 ° C , maximum activity of 5.89 IU / mL, than that before optimization nearly 2 -fold . The medium formula strains HDDMG05 citrus peel powder 16.1% , yeast extract 0.2 % , 0.5% NaCl , K 2 the HPO 4 0.5% MgSO 4 < / sub> .7 H 2 O 0.01%, culture conditions , inoculum size 2% , temperature 37 ° C maximum activity 8126U/mL, and 16 times more than that before optimization . Through the determination of the chemical composition of hemp fibers , determine the best retting laboratory conditions to cultivate : Breeding the strain HDDMG05 and HDDMM02 is to the maximum period of enzyme production the two inoculation were 0.5% , a ratio of 1:1 , , additives urea 0.1%, NaCl 0.1% . Retting hemp fiber in pectin , hemicellulose and lignin content were 5.32% , 17.96% and 2.11% , a decrease of 1-3 percentage points compared to which all without bacteria . Average out of the factory plus bacteria test hemp fiber hemp was 13.11% and the average bacteria before Ma was 11.66% . Plus bacteria out Ma 1-1.5 % hemp fiber strength of 20-25N ( Newton ) .

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CLC: > Agricultural Sciences > Crop > Economic crops > Fiber crops > Fiber crops > Cannabis ( white hemp, Marijuana )
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