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The Study of the Therapeutic Alliance of AAV-AS and Cytoxan on C6 Glioma in Rats

Author: WangGuan
Tutor: HuangZuo；HuangHuiLing；FengZuoZuo
School: Tianjin Medical University
Course: Surgery
Keywords: Adeno-associated virus vector angiostatin recombinant gene Cyclophosphamide Glioma Antiangiogenic Combination therapy Apoptotic index
CLC: R739.41
Type: Master's thesis
Year: 2009
Downloads: 21
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Abstract


Objective: application adeno -associated virus carrier the angiostatin recombinant gene (AAV-AS) in combination with cyclophosphamide (CTX) treatment of rat subcutaneous C6 glioma observed tumor volume , neovascularization and apoptosis explore antiangiogenic gene therapy combined with chemotherapy for glioma treatment prospects . Methods: Dutch rat C6 glioma subcutaneous transplantation model , when the mean tumor volume after 7d 250 ~~ 280mm 3 , were randomly divided into four groups , and were given saline the CTX AAV- AS , and CTX, AAV- AS the combination treatment of every length of 3d measurement of tumor , short diameter and calculate volume , at 22d after put to death at the same time , detection the AS transcription , vascular density and tumor cell apoptosis . Results : The combined therapy group and AAV-AS treatment group were detected to the AS protein , gene transduction successful ; combination therapy 22d tumor volume (1098.320 ± 70.880mm ~ 3) and the control group ( 2060.339 ± 82.028mm ~ 3 ) , chemotherapy group ( 1763.867 of ± 124.237mm to 3 ) and AAV-AS treatment group compared to the (1229.910 ± 141.646mm ~ 3) were statistically significant (P lt; 0.05), tumor volume was significantly reduced ; vascular density test showed that the combined treatment group (8.364 ± 3.572/0.24mm ~ 2) and the control group ( 19.810 ± 3.423/0.24mm ~ 2 ) , chemotherapy group (17.737 ± 4.658/0.24mm ~ 2) and AAV-AS treatment group ( 12.631 ± 2.359/0.24mm to 2 ) were statistically significant ( P lt; 0.05 ) compared blood vessel growth was significantly inhibited ; apoptotic index AI detection showed that the combined treatment group ( 3.886 ± 0.381 ) and control group (1.442 ± 0.776), chemotherapy group compared to (1.719 ± 0.344) were statistically significant (P lt; 0.05), indirectly promote tumor cell apoptosis . Conclusion: Gene therapy combined with chemotherapy can inhibit glioma angiogenesis and inhibit tumor growth, combined with gene therapy with traditional chemotherapy drugs , the synergies which play a combination of both , and to make up for the inadequacies of the two alone .

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CLC: > Medicine, health > Oncology > Nervous system tumors > Intracranial tumors and brain tumors
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