Dissertation > Excellent graduate degree dissertation topics show

Study on the Correlation between Calbindin-D9k and Antiimplantation Effect of Methoxychlor in the Mouse

Author: ZuoYunGuo
Tutor: MaXiangDong
School: Fourth Military Medical University
Course: Obstetrics and Gynaecology
Keywords: Environmental estrogens Methoxychlor Embryo implantation Calcium-binding protein D9k Early pregnancy Mice Estrogen receptor alpha subunit Progesterone receptor
CLC: R114
Type: Master's thesis
Year: 2009
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


The environmental estrogen hazards has aroused great concern. Many studies have shown that environmental estrogens on animals have endocrine-disrupting effects, can significantly reduce the reproductive function [1,2]. Methoxychlor (MXC) is an alternative to the organochlorine insecticide DDT in the body can be converted to metabolites with estrogenic activity [3]. Is widely used to kill the insect pests of crops and indoor. Compared with DDT, MXC has low toxicity, rapid metabolism, is not easy to accumulate in the fatty tissue, etc. [4]. The calcium-binding protein D9k (Calbindin-D9k) with a molecular weight of a 9kD the calcium-binding protein, belongs to a family of proteins within the cell. Uterine Calbindin-D9k is closely related with the level of intracellular calcium, that can control the activity of the myometrium [8]. In addition, there is evidence that in the early stages of embryo implantation necessary Calbindin-D9k it successfully implanted embryos play a crucial role [9]. The study found that immature female rats exposed to the environmental estrogen as MXC, can cause the uterus Calbindin-D9k expression increased significantly and dose-dependent manner, so Calbindin-D9k detection can be used as an environmental estrogen-like chemicals estrogen biomarkers of hormone activity, however, research on MXC embryo implantation Calbindin-D9k expression is scarce. The purpose of this subject is by using the MXC exposed pregnant mice as an experimental animal model to investigate the effects of MXC pregnant mouse uterine estrogen receptor α subtypes (ERα), progesterone receptor (PR) expression and mice pregnancy rate and the number of embryo implantation. At the same time, further in-depth study MXC to exposed pregnant mouse uterine Calbindin-D9k mRNA and protein expression, reveals MXC inhibit the molecular mechanism of embryo implantation. The experiment was divided into two parts. Part I: Building the MXC exposed pregnant mouse model to observe the influence of MXC for implantation of the embryo and the uterus of ERα, PR expression. Eighty mating success of female mice were randomly assigned to four groups (n = 20). 3 MXC treated mice from pregnant 1d (found vaginal plug as pregnant 0d) from the given MXC gavage once daily, continuous 3d, at doses as low-dose group (100 mg / kg), in dose group (200 mg / kg), high dose group (400mg/kg), MXC dissolved in sesame oil administration. The control group of mice only the solvent filling sesame. To pregnant 5d killed pregnant rats were quickly isolated uterus of mice pregnancy rate and the number of embryo implantation. Uterus of ERα, PR, mRNA expression, immunohistochemistry and image analysis to detect the uterus of ERα, PR, protein expression was detected by RT-PCR. Results the: ① pregnant mice MXC gavage for 3 d, high dose MXC group of pregnant mice pregnancy rate compared with the control group significantly reduce (P lt; 0.05), low dose MXC group of pregnant mice pregnancy rate no significant difference compared with the control group reduced; ②, compared to the average dose MXC group of pregnant mice embryo implantation and the control group was significantly lower (P lt; 0.01), however, the low-dose group of pregnant mice embryo implantation Although the number has decreased, but not statistically significant. ③ With the MXC doses of the increase, the pregnant mice the uterus ERαmRNA with the amount of expression of the protein corresponding increase high in the dose group ERαmRNA protein expression compared with the control group, the difference was statistically significance (P lt; 0.05) ; ④ uterine PR mRNA expression in pregnant mice of the middle dose group than in the control group significantly reduce (P lt; 0.05), the low-dose group PR mRNA expression was no significant change the PR protein change with PRmRNA of change consistent. The second part: MXC trimester Calbindin-D9k gene expression in mouse uterus affect experimental animal model with experimental exposure. Calbindin-D9k mRNA expression was detected by RT-PCR, Western blot and immunohistochemical staining Calbindin-D9k protein expression. Results: When pregnant rats exposed to MXC 3 d Calbindin-D9k mRNA expression levels in the medium dose group compared with the control group was significantly lower (P lt; 0.05), however, Calbindin-D9k mRNA expression of the low-dose group with control The group was not changed significantly (P gt; 0.05) compared. Calbindin-D9k protein expression in endometrial epithelial cells and glandular epithelial cells in the pregnant 5 d. Corresponding mRNA expression levels Calbindin-D9k protein expression levels in the medium dose group compared with the control group was significantly lower (P lt; 0.05), while the low-dose group compared with the control group, no significant difference (P gt; 0.05 ). Conclusion: Based on the above results, we draw the following conclusions: ① MXC exposure of pregnant mice can significantly reduce the pregnancy rate and the number of embryo implantation, was positively correlated with inhibition of the embryonic implantation of MXC doses of. ② MXC raised the pregnant the mouse uterus ERαmRNA and the protein expression by its weak estrogenic activity and antagonistic progesterone, while reduced PR mRNA and protein expression, MXC can interfere with the pregnant mice estrogen, progesterone normal environment, inhibition of embryo implantation . ③ embryo implantation early Calbindin-D9k involved in the regulation of implantation sites calcium utilization and control of the activities of the myometrium, to play an important role in stability implantation of the embryo. MXC exposure to decrease of implantation pregnant mice uterine Calbindin-D9k mRNA and protein expression, its lowered level of and MXC exposure dose was positively correlated. The ④ MXC exposure lowered pregnant mice uterus Calbindin-D9k expression of PR mRNA and protein expression decreased confirmed the mouse uterine Calbindin-D9k the pregnancy hormone regulation theory, MXC reduce the pregnant mouse uterus Calbindin-D9k expression may be the an important part of the mechanism of inhibition of embryo implantation.

Related Dissertations

  1. Study of Sepia Ink-Astragalus Preparation for Relieving Damage Caused by Chemotherapy,R285.5
  2. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  3. Study on Gene Expression of β-defensin1 in Mammary Gland of Mice,S852.4
  4. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  5. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  6. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  7. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329
  8. Effects of TACI-Ig on MRL/lpr Mice and Activation of JAK1-STAT1 Signaling Pathway in Lupus Nephritis,R363
  9. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  10. Maternal Exposure to Fenvalerate in Embryo Accelerated Age-related Changes of Behaviors in CD-1 Mice,R714.2
  11. Development of the Colloidal Gold Immuno-Chromatographyic Strips for the Early Pregnancy Diagnosis of Hu Sheep,S858.26
  12. The Experiment Study Was Used Three Methods to Repair the Ligation of the Femoral Nerve,R651.3
  13. Effect of β-elemene Combined with Radiotherapy on the Angiogenesis of Lung Adenocarcinoma Xenograft Tumor in Nude Mice,R734.2
  14. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  15. The Experimental Research of RNAi Technology Inhibiting Respiratory Syncytial Virus,R725.6
  16. The Correlation of Ultrasound Elasticity with the Expressions of Estrogen Receptor, Progestron Receptor, C-erbB-2 in Breast Cancer,R445.1
  17. A Stuty on the Diversification of Interleukin-17 of Asthma Mouse and the Affection of the Treament of Glucocorticoids,R562.25
  18. Influences of Cisplatin on Expressions of P-glycoprotein in the Resistance Cells of in Lung Cancer Xenografts in the Mice,R734.2
  19. Study on the in Vitro Effect of Zinc on the Growth of Murine Embryonic Palatal,R782.2
  20. Taxifolin , quercetin on LPS-induced abortion mouse macrophages affect the uterus,R285.5
  21. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9

CLC: > Medicine, health > Preventive Medicine,Health > Health - basic science > Health Toxicology
© 2012 www.DissertationTopic.Net  Mobile