|
Micro-column liquid chromatography with a high separation efficiency , analysis speed, less mobile phase consumption , environmental pollution, etc. , are widely used in biochemical analysis , food and environmental testing and neurological sciences. For routine analytical instruments to be improved, to construct micro-column liquid chromatography system . The constructed system has good stability , high sensitivity to standard samples according to Bartlett inverting the sample , seven consecutive injections analysis, retention time RSD values ??< 0.14% , peak area RSD values ??< 1.1% , to meet the qualitative liquid chromatography and quantitative requirements. Micro-column liquid chromatography using gradient elution mode , 15min to complete separation of the seven kinds of aromatic compounds . Analytical column with 4.6mmI.D. analysis , it is found in column efficiency is essentially the same in the case , 1.0mmI.D. micro column analysis speed has been greatly improved . At the same linear velocity of the mobile phase consumption was reduced by 96%. The micro-column liquid chromatography system is applied to the separation and analysis of real samples , the development of melamine in food , benzoic acid and sorbic acid liquid chromatography analysis of methods for all components , retention time RSD values ??< 0.30% , peak area RSD values < 0.70% , the linear correlation coefficient > 0.999 , the detection limit (S / N = 3) were 0.015mg/kg, 0.077mg/kg, 0.084mg/kg. The system also applies Prunella , polycyclic aromatic hydrocarbons and biogenic amines analysis of samples with good results . Micro-column liquid chromatography system and its evaluation , for promoting their industrialization , the development of appropriate practical method of guiding significance .
|